Product nameHuman MFGE8 Antibody Pair - BSA and Azide free
See all Milk Fat Globule 1 kits
Assay typeELISA set
Range15.6 pg/ml - 2000 pg/ml
Species reactivityReacts with: Human
The Antibody Pair can be used to quantify Human MFGE8. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab235638), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
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Tested applicationsSuitable for: Sandwich ELISAmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 10 x 96 tests Human MFGE8 Capture Antibody (unconjugated) 1 x 100µg Human MFGE8 Detector Antibody (unconjugated) 1 x 100µg
FunctionPlays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.
Medin is the main constituent of aortic medial amyloid.
Tissue specificityMammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.
Sequence similaritiesContains 1 EGF-like domain.
Contains 2 F5/8 type C domains.
modificationsMedin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.
Cellular localizationMembrane. Secreted.
- Information by UniProt
- Breast epithelial antigen BA46
Our Abpromise guarantee covers the use of ab244126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
To learn more about the advantages of recombinant antibodies see here.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244126 has not yet been referenced specifically in any publications.