Overview

  • Product name

    Human MFGE8 ELISA Kit (Lactadherin)
    See all Milk Fat Globule 1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 669pg/ml 42.82 6.4%
    2 16 1295pg/ml 58.3 4.5%
    3 16 2234pg/ml 116.2 5.2%
    Inter-assay
    Sample n Mean SD CV%
    1 24 724pg/ml 56.5 7.8%
    2 24 1443pg/ml 86.58 6%
    3 24 2458pg/ml 135.2 5.5%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Heparin Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human MFGE8 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213810) is designed for the quantitative detection of Human MFGE8 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. 


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for MFGE8 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: L24-C387) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MFGE8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MFGE8 amount of sample captured in plate. 

  • Notes

    MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, the MFGE8 gene was mapped to chromosome 15q25. MFGE8 is a factor that links apoptotic cells to phagocytes. It specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. It showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human MFGE8 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human MFGE8 antibody 1 x 130µl
    Lyophilized recombinant Human MFGE8 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.
    Medin is the main constituent of aortic medial amyloid.
  • Tissue specificity

    Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.
  • Sequence similarities

    Contains 1 EGF-like domain.
    Contains 2 F5/8 type C domains.
  • Post-translational
    modifications

    Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.
  • Cellular localization

    Membrane. Secreted.
  • Information by UniProt
  • Alternative names

    • BA46
    • Breast epithelial antigen BA46
    • EDIL1
    • HMFG
    • hP47
    • HsT19888
    • Lactadherin
    • Medin
    • MFG-E8
    • MFGE8
    • MFGM
    • MFGM_HUMAN
    • Milk fat globule EGF factor 8
    • Milk fat globule EGF factor 8 protein
    • Milk fat globule-EGF factor 8
    • O acetyl disialoganglioside synthase
    • OAcGD3S
    • SED1
    • SPAG10
    • Sperm associated antigen 10
    • Sperm surface protein hP47
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human MFGE8 ELISA Kit (Lactadherin) (ab213810) Standard Curve

Protocols

References

ab213810 has not yet been referenced specifically in any publications.

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