Overview

  • Product name
    Human MICA ELISA Kit
    See all MICA kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    13.72 pg/ml - 10000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 116.9 109% - 124%
    Serum 112.3 95% - 129%
    Plasma 93.83 75% - 107%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s MICA Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human MICA in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MICA coated on a 96-well plate. Standards and samples are pipetted into the wells and MICA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MICA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MICA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    Biotinylated anti-Human MICA (lyophilized) 2 vials
    MICA Microplate (12 x 8 wells) 1 unit
    Recombinant Human MICA Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    The MHC class I chain-related (MIC) proteins are related to the Major histocompatibility complex (MHC) class I proteins which are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. The MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. MICA is present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.
  • Cellular localization
    Plasma membrane
  • Alternative names
    • FLJ60820
    • HLA class I antigen
    • MGC111087
    • MHC class I chain related gene A protein
    • MHC class I chain related protein A
    • MHC class I polypeptide related sequence A
    • MHC class I related protein
    • MIC A
    • PERB11.1
    • Stress inducible class I homolog
    see all
  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab100592 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • ELISA: MICA Human ELISA Kit (ab100592)
      ELISA: MICA Human ELISA Kit (ab100592)

      Standard curve with background signal subtracted (duplicates; +/- SD).

    • ELISA: MICA Human ELISA Kit (ab100592)
      ELISA: MICA Human ELISA Kit (ab100592)

      MICA measured in undiluted cell culture supernatants, U937 and THP-1 signals were below level of detection (13.7 pg x mL-1) (duplicates +/- SD).

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab100592.

    Protocols

    References

    This product has been referenced in:
    • Ren J  et al. Estrogen upregulates MICA/B expression in human non-small cell lung cancer through the regulation of ADAM17. Cell Mol Immunol N/A:N/A (2014). Read more (PubMed: 25363527) »

    See 1 Publication for this product

    Publishing research using ab100592? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 reviews or Q&A

    10
    Votes: Highest First

    1) Extracellular domain of MICA not detected. Is the standard the recombinant full length MICA protein or a fragment?
    2) Standard values are much lower than the ones obtained with a kit from a competitor. The values are ok when the standard from th...

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    Thank you for contacting us.

    Please find below the answers from our collaborator to your questions:

    1) Extracellular domain of MICA not detected. Is the standard the recombinant full length MICA protein or a fragment?
    The antibody...

    Read More

    Dear tech,   A customer would like to purchase a ELISA kit for soluble MICA. Do ab100592 and ab59569 match the need? Thank you for your help!   Best regards,

    Thank you for your patient. Both products are suitable to use with soluble MICA.  However, please notice there are slightly differences between the kits. Ab100592 has been tested in human fluids, as Urine, Serum, and Plasma and also in Cell culture sup...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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