Overview

  • Product name

    Human MIP2 ELISA Kit, Fluorescent
    See all CXCL2 kits
  • Detection method

    Fluorescent
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Media 24 2.81%
    Inter-assay
    Sample n Mean SD CV%
    Media 24 3.46%
  • Sample type

    Serum, Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.26 pg/ml
  • Range

    0.29 pg/ml - 300 pg/ml
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Rabbit, Goat, Guinea pig, Hamster, Cow, Dog, Pig
  • Product overview

    MIP2 (CXCL2) in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of MIP2 (CXCL2) protein in human serum, plasma and cell culture supernatants.


    This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
    If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.


    The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material.  CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

  • Notes

    Macrophage inflammatory protein 2 (MIP2), otherwise known as CXCL2, GRO-beta, or Hematopoietic synergistic factor, is a 7.9 kDa heparin-binding chemokine that has potent effects in the response to inflammation and induction of peripheral tolerance. It is secreted by activated monocytes, neutrophils and inflamed mucosal epithelial cells in response to inflammatory stimuli such as IL-1β. MIP2 recruits granulocytic neutrophils and macrophages at sights of inflammation, and causes degranulation of these effector cells at the inflammatory site.  It has also been hypothesized that MIP2 acts to synergize the effects of Granulocyte macrophage colony-stimulating factor (GM-CSF) and Macrophage colony-stimulating factor (M-CSF), leading to a larger recruitment of neutrophils and macrophages at the site of inflammation.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Stoplight Red Substrate 1 x 12ml
    10X Human MIP2 Capture Antibody 1 x 600µl
    10X Human MIP2 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl
    Antibody Diluent 4BI 1 x 6ml
    Human MIP2 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 25BP 1 x 20ml
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated Black 96-Well Microplate 1 unit
    Stoplight Red Substrate Buffer 1 x 12ml
  • Research areas

  • Function

    Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • C-X-C motif chemokine 2
    • Chemokine (C X C motif) ligand 2
    • Chemokine, CXC motif, ligand 2
    • CINC 2a
    • CINC2a
    • CINC3
    • CXC chemokine
    • CXCL 2
    • Cxcl2
    • CXCL2_HUMAN
    • Cytokine-induced neutrophil chemoattractant 3
    • GRO 2
    • GRO b
    • GRO protein, beta
    • Gro-beta
    • GRO-beta(5-73)
    • GRO-beta-T
    • GRO2
    • GRO2 oncogene
    • GROb
    • GRObeta
    • Growth regulated protein beta
    • Growth-regulated protein beta
    • GROX
    • Hematopoietic synergistic factor
    • HSF
    • Macrophage inflammatory protein 2
    • Macrophage inflammatory protein 2 alpha
    • Macrophage inflammatory protein 2-alpha
    • Melanoma growth stimulatory activity beta
    • MGSA b
    • MGSA beta
    • MIP 2
    • MIP 2a
    • MIP2
    • MIP2 alpha
    • MIP2-alpha
    • MIP2A
    • MIP2alpha
    • SB-251353
    • Scyb
    • SCYB 2
    • SCYB2
    • Small inducible cytokine subfamily B, member 2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab229427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Samples were prepared according to linearity of dilution section described in Typical Sample Values section of the protocol.  Interpolated values corrected by dilution factor (mean +/- SD) are graphed.

  • Human PBMCs were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL penicillin and 100 µg/mL streptomycin.  Cells were cultured for 2 days at 37˚C in the presence or absence of PHA.  The concentrations of MIP2 were interpolated from the calibration curve and corrected for sample dilution.  The mean MIP2 concentration was 67 pg/mL on unstimulated PBMC supernatants and 773 pg/mL on stimulated PBMCs supernatants.

  • Ten individual healthy donors were evaluated for the presence of MIP2 in serum using this assay. Results were interpolated from the standard curve in Sample Diluent 25BP and corrected for sample dilution (1:4). The mean level of Human MIP2 was found at 84.757 pg/mL with a range of 26.767 – 266.256 pg/mL.

Protocols

References

ab229427 has not yet been referenced specifically in any publications.

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