• Product name
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 7 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 93.48 81% - 105%
    Serum 92.78 82% - 105%
    Plasma 94.16 83% - 106%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s MIP3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP3 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MIP3 coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    Get higher sensitivity in only 90 minutes with Human MIP3 ELISA Kit (ab216169) from our SimpleStep ELISA® range.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human MIP3 2 vials
    MIP3 Microplate (12 x 8 wells) 1 unit
    Recombinant Human MIP3 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23.
  • Tissue specificity
    High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are found in high levels in synovial fluids from rheumatoid patients.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • C C motif chemokine ligand 23
    • C-C motif chemokine 23
    • C6 beta chemokine
    • CCL 23
    • CCL23
    • CCL23(30-99)
    • CCL23_HUMAN
    • Chemokine (C C motif) ligand 23
    • Chemokine CC motif ligand 23
    • ck beta 8
    • CK-beta-8
    • CKB 8
    • CKB-8
    • Ckb-8-1
    • CKB8
    • Hmrp-2a
    • Macrophage inflammatory protein 3
    • MIP 3
    • MIP-3
    • MIP3
    • MPIF 1
    • MPIF-1
    • MPIF1
    • Myeloid progenitor inhibitory factor 1
    • OTTHUMP00000163948
    • SCYA 23
    • SCYA23
    • Small inducible cytokine A 23
    • Small inducible cytokine subfamily A (Cys-Cys), member 23
    • Small inducible cytokine subfamily A, member 23
    • Small-inducible cytokine A23
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab100611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab100611.

  • Representative Standard Curve using ab100611.



ab100611 has not yet been referenced specifically in any publications.

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