Human MiTF peptide (ab21454)
- Datasheet
- References
- Protocols
Overview
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Product nameHuman MiTF peptide
See all MiTF proteins and peptides
Description
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NatureSynthetic
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Amino Acid Sequence
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SpeciesHuman
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Associated products
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Corresponding Antibody
Specifications
Our Abpromise guarantee covers the use of ab21454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Blocking - Blocking peptide for Anti-MiTF antibody (ab20663)
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FormLiquid
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Additional notes
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
General Info
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Alternative names
- BHLHE32
- bHLHe32
- Class E basic helix-loop-helix protein 32
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FunctionTranscription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
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Tissue specificityIsoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells.
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Involvement in diseaseDefects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete. -
Sequence similaritiesBelongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsPhosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter. -
Cellular localizationNucleus.
- Information by UniProt
Images
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Western blot - Human MiTF peptide (ab21454)All lanes : Anti-MiTF antibody (ab20663) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa nuclear lysate
Lane 3 : A431 whole cell lysate
Lane 4 : HeLa whole cell lysate withHuman MiTF peptide (ab21454) at 1 µg/ml
Lane 5 : HeLa nuclear lysate withHuman MiTF peptide (ab21454) at 1 µg/ml
Lane 6 : A431 whole cell lysate withHuman MiTF peptide (ab21454) at 1 µg/ml
Lysates/proteins at 1 µg/ml per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
References
ab21454 has not yet been referenced specifically in any publications.
