Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6.29 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman MLKL ELISA Kit
See all MLKL kits
Intra-assay Sample n Mean SD CV% Extract 8 10.2% Inter-assay Sample n Mean SD CV% Extract 3 6.8%
Sample typeCell Lysate
Assay typeSandwich (quantitative)
Range78.13 pg/ml - 5000 pg/ml
Sample specific recovery Sample type Average % Range Cell Lysate 90 82% - 100%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Human MLKL ELISA Kit (ab263892) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MLKL protein in cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MLKL with 6.29 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mixed lineage kinase domain-like (MLKL) is a pseudokinase containing a protein kinase-like domain that plays a critical role in tumor necrosis factor (TNF)-induced necroptosis as a functional substrate of receptor-interacting protein kinase 3 (RIP3). MLKL, together with receptor-interacting protein 1 (RIP1) and RIP3, form a necrosis signaling complex termed the necrosome, within which MLKL is phosphorylated at T357 and S358 residues by RIP31. Once phosphorylated, MLKL oligomers localize to the plasma membrane and form disrupting pores, capable of regulating the influx of Na+ and Ca2+ rupturing the cell membrane. Necroptosis requires MLKL and RIP3 and is implicated in the pathogenesis of various diseases through its role in necroptosis. Human MLKL shares 69% and 68% sequence homology with mouse and rat, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human MLKL Capture Antibody 1 x 600µl 10X Human MLKL Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human MLKL Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
Sequence similaritiesBelongs to the protein kinase superfamily.
Contains 1 protein kinase domain.
DomainThe protein kinase domain is predicted to be catalytically inactive.
- Information by UniProt
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
The MLKL standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of MLKL were measured in duplicate and interpolated from the MLKL standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MLKL concentration was determined to be 741.83 pg/mL in HUVEC cell extract.
To learn more about the advantages of recombinant antibodies see here.
ab263892 has not yet been referenced specifically in any publications.