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  1. Link

    human-mmp-antibody-array-membrane-10-targets-ab134004.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases TIMP
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Human MMP Antibody Array - Membrane (10 Targets) (ab134004)

  • Datasheet
  • SDS
  • Protocol Booklet
Q&A (2)References (11)

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Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)
  • Quantification of Human MMP Antibody Array
  • Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)
  • Quantification of Human MMP Antibody Array
  • Dot Blot - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)

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Overview

  • Product name

    Human MMP Antibody Array - Membrane (10 Targets)
    See all MMP Antibody Array antibody arrays
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type

    Semi-quantitative
  • Species reactivity

    Reacts with: Human
  • Product overview

    ab134004 is for simultaneous detection of 7 Human MMPs and 3 TIMPs. Suitable for all sample types.


    Targets: MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, TIMP-4


    Antibody arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The antibody array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


    Learn more about membrane antibody arrays

  • Notes

    If you are interested in this antibody array, array ab169816 may also be of interest.


    A table of human antibody membrane arrays and the analytes they measure is available here.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Tested applications

    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes
    1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl
    20X Wash Buffer I 1 x 10ml 1 x 20ml
    20X Wash Buffer II 1 x 10ml 1 x 20ml
    2X Cell Lysis Buffer 1 x 10ml 1 x 16ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit
    Biotin-Conjugated Anti-Cytokines 2 vials 4 vials
    1X Blocking Buffer 1 x 25ml 2 x 25ml
    Cytokine Antibody Array Membranes 4 units 8 units
    Detection Buffer C 1 x 1.5ml 1 x 2.5ml
    Detection Buffer D 1 x 1.5ml 1 x 2.5ml
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Matrix Metalloproteinases
    • TIMP
    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Matrix Metalloproteinases
    • MMP
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • MMPs
    • Antibody Arrays
    • Membrane antibody arrays
    • EGFR phosphorylation antibody arrays
    • Antibody Arrays
    • Membrane antibody arrays
    • Matrix metalloproteinase antibody arrays

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection
Use at an assay dependent concentration.
Notes
Multiplex Protein Detection
Use at an assay dependent concentration.

Images

  • Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)
    Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)

    Human serum and plasma from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134004.

  • Quantification of Human MMP Antibody Array
    Quantification of Human MMP Antibody Array

    Human serum and plasma from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134004. Mean pixel density was quantified using CCD camera software analysis.

  • Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)
    Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)

    143B cells (Human osteosarcoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate.

    Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134004.

  • Quantification of Human MMP Antibody Array
    Quantification of Human MMP Antibody Array

    Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134004. Mean pixel density was quantified using CCD camera software analysis.

  • Dot Blot - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)
    Dot Blot - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)This image is courtesy of an anonymous customer.
    Membranes were probed with media and cell lysate from control and treated MCF-7 cells. These membranes were exposed to film for 1 minute.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (11)

Publishing research using ab134004? Please let us know so that we can cite the reference in this datasheet.

ab134004 has been referenced in 11 publications.

  • Triebel J  et al. Matrix Metalloproteases and Cathepsin D in Human Serum do not Cleave Prolactin to Generate Vasoinhibin. Clin Lab 66:N/A (2020). PubMed: 32390374
  • Dias F  et al. Plasma Extracellular Vesicle-Derived TIMP-1 mRNA as a Prognostic Biomarker in Clear Cell Renal Cell Carcinoma: A Pilot Study. Int J Mol Sci 21:N/A (2020). PubMed: 32610589
  • Cathery W  et al. Umbilical Cord Pericytes Provide a Viable Alternative to Mesenchymal Stem Cells for Neonatal Vascular Engineering. Front Cardiovasc Med 7:609980 (2020). PubMed: 33553259
  • Balta E  et al. Spatial oxidation of L-plastin downmodulates actin-based functions of tumor cells. Nat Commun 10:4073 (2019). PubMed: 31501427
  • Lye JJ  et al. Astrocyte senescence may drive alterations in GFAPa, CDKN2A p14ARF, and TAU3 transcript expression and contribute to cognitive decline. Geroscience 41:561-573 (2019). PubMed: 31654269
View all Publications for this product

Customer reviews and Q&As

Q&A
Submit a question

1-2 of 2 Q&A

Question

Thank you very much for sending the HRP-Streptavidin. It will allow me to work on the remaining 3 membranes.

I was wondering if you had heard anything about the price of detections buffers C and D.

I would like to begin this experiment fairly soon and ordering it would take time I think.

I look forward to hearing from you soon.

Kind regards,

Read More

Abcam community

Verified customer

Asked on Jan 30 2013

Answer

Thank you for your email.

We can provide the buffer C and D at £50 each or £100 for both. Please let me know if you are interested in buying these I will then ask my colleague to add these in your order.

Thanks!

Read More

Abcam Scientific Support

Answered on Jan 30 2013

Question

The HRP strepatavidin is only 6ul where it shold be 16ul.

Read More

Abcam community

Verified customer

Asked on Jan 21 2013

Answer

Thank you very much for contacting us and alerting us with this issue.

I can confirm that I have started further step to replace the component you have received.

Please keep me informed about the results.

Many thanks for your cooperation in this case.

Read More

Abcam Scientific Support

Answered on Jan 21 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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