Human MMP Antibody Array - Membrane (10 Targets) (ab134004)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameHuman MMP Antibody Array - Membrane (10 Targets)
See all MMP Antibody Array antibody arrays -
Sample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
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Assay typeSemi-quantitative
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Species reactivityReacts with: Human
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Product overview
ab134004 is for simultaneous detection of 7 Human MMPs and 3 TIMPs. Suitable for all sample types.
Targets: MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, TIMP-4
Antibody arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The antibody array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
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Notes
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Tested applicationsSuitable for: Multiplex Protein Detectionmore details
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Streptavidin Buffer 1 x 50µl 1 x 50µl 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Biotin-Conjugated Anti-Cytokines 2 vials 4 vials 1X Blocking Buffer 1 x 25ml 2 x 25ml Cytokine Antibody Array Membranes 4 units 8 units Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml -
Research areas
Applications
Our Abpromise guarantee covers the use of ab134004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Multiplex Protein Detection | Use at an assay dependent concentration. |
Images
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Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)Human serum and plasma from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134004.
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Quantification of Human MMP Antibody ArrayHuman serum and plasma from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134004. Mean pixel density was quantified using CCD camera software analysis.
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Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)143B cells (Human osteosarcoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate.
Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134004.
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Quantification of Human MMP Antibody ArrayCells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134004. Mean pixel density was quantified using CCD camera software analysis.
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Dot Blot - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)This image is courtesy of an anonymous customer.Membranes were probed with media and cell lysate from control and treated MCF-7 cells. These membranes were exposed to film for 1 minute.
Protocols
References
This product has been referenced in:
- Hohensinner PJ et al. PAI-1 (Plasminogen Activator Inhibitor-1) Expression Renders Alternatively Activated Human Macrophages Proteolytically Quiescent. Arterioscler Thromb Vasc Biol N/A:N/A (2017). Read more (PubMed: 28818858) »
- Baig MS et al. Non-canonical role of matrix metalloprotease (MMP) in activation and migration of hepatic stellate cells (HSCs). Life Sci 155:155-60 (2016). Read more (PubMed: 27140333) »
