Overview

  • Product name

    Human MMP1 ELISA Kit
    See all MMP1 kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 8 pg/ml
  • Range

    24.69 pg/ml - 18000 pg/ml
  • Recovery

    98 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.66 90% - 106%
    Serum 97.78 88% - 106%
    Plasma 99.59 90% - 107%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Product overview

    Human MMP1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP1 pro and active forms in serum, plasma (using heparin as an anticoagulant, EDTA and Citrate are not recommended), and cell culture supernatants.


    This assay employs an antibody specific for Human MMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • MMP1 measured in cell culture medium with background signal subtracted (duplicates +/- SD).

  • MMP1 measured in biological fluids with background signal subtracted (duplicates +/- SD).

  • Representative Standard Curve using ab100603.

Protocols

References

This product has been referenced in:

  • Meng D  et al. Effects of VEGFR1+ hematopoietic progenitor cells on pre-metastatic niche formation and in vivo metastasis of breast cancer cells. J Cancer Res Clin Oncol 145:411-427 (2019). Read more (PubMed: 30483898) »
  • Park SH  et al. Protection against UVB-induced damages in human dermal fibroblasts: efficacy of tricin isolated from enzyme-treated Zizania latifolia extract. Biosci Biotechnol Biochem N/A:1-10 (2018). Read more (PubMed: 30526376) »
See all 8 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Nous ne recommandons pas de modifier les concentrations des courbes standards mais plutôt de diluer vos échantillons.

Toutefois, si vous voulez vraiment changer les courbes standards ceci est possible. Cependant en augmentant les concentrations de la courbe standard, vous augmentez considérablement le risque de saturer l'anticorps qui peut conduire à un effet de plafonnement (une courbe non linéaire). N’oublions pas que l’anticorps de capture a aussi une limite du taux de protéine qu’il peut capturer.

Veuillez noter que beaucoup de tests ont été réalisé pour déterminer les concentrations standards optimales pour les anticorps et donc si vous décidez de modifier les gammes de standards nous ne pourrons plus garantir que ces produits fonctionneront.

De plus, nous ne savons pas à partir de quelle concentration les standards sont solubles.

Pour ab100603, nous recommandons de reconstituer le standard dans 400 ul de diluant pour faire 100 ng/ml. Ceci est après dilué ˜5.555 fois pour faire une concentration de 18,000 pg/ml. Je vous conseille donc de diluer le standard de 100 ng/ml moins que 5.55 fois pour obtenir une gamme plus concentré.

Pour ab46032, nous ne recommandons pas de reconstituer le standard dans un plus petit volume, pour obtenir 4.000 pg/mL.

Read More

Answer

Thank you for your inquiry.

For ab100603 lot GR47531-1, it was entered into stock in June 2011. We set an expiration guidelinefor 1 year from that point, so June 2012.

For ab100651 lot GR49880-1 was put into stock in July, Aug and Oct 2011. We set an expiration guideline for July 2012.

I hope this information helps. Please contact us with any other questions.

Read More

Answer

Thank you and your customerfor taking the time to complete our questionnaire and contact us. I am sorry to hear your customerhas had difficulty obtaining satisfactory results from this antibody.

The details your customerhas kindly provided will provide us with vital information for our monitoring of product quality.

I appreciate the time your customerhas spent in the laboratory and understandtheir concerns. It is regrettable the results have not been successful. I can suggest your customerhas regrettably received bad kits.

I apologise for the inconvenience and am pleased to offer your customera free of charge replacement or credit note in compensation.

In addition, I can suggest your customer may like to consider the following suggestions for future experiments:

Please let us know if there precipitation before using the kit. If the customer find precipitation, we are suggesting to spin the buffer down and only use the supernatant. Or alternatively filter the buffer.

Thank you and your customerfor your cooperation. I look forward to hearing from you with details of how your customerwould like to proceed.

Read More

Answer

Thank you for contacting Abcam.

Unfortunately, to our knowledge ab100603, ab100607, and ab100605 have not been tested with monkey samples so there is no cross reactivity data available. However, I believe that MMPs are very well conserved among species so I would expect the degree of homology between human and monkey to be quite high. Therefore, I think it would be worth a shot as the only way to know at this point would be to test.

As these kits have not been tested in monkey, I can offer you our 100% Abreview Testing Discount. Please see the link below for more information on the program (your free product would be a kit):

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



If it helps, the capture and detection antibodies in all three of these kits are a monoclonal and polyclonal respectively. Also, all of the kits detect the pro and mature form of the protein.

Please let me know if you have any questions or if you would like to take advantage of the testing discount program.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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