Overview

  • Product name

    Human MMP13 ELISA Kit
    See all MMP13 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Supernatant 8 5.9%
    Inter-assay
    Sample n Mean SD CV%
    Supernatant 3 5%
  • Sample type

    Saliva, Urine, Serum, Cell culture media
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    5.7 pg/ml
  • Range

    39 pg/ml - 2500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 80 78% - 82%
    Urine 84 74% - 93%
    Serum 83 78% - 88%
    Cell culture media 102 101% - 103%

  • Assay time

    2h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Rat, Rabbit, Cow, Human, Pig
    Does not react with: Mouse
  • Product overview

    MMP-13 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human MMP-13 protein in serum, urine, saliva, and cell culture supernatants.


    The ELISA employs an affinity tag labeled capture antibody, a biotin conjugated detector antibody and a Streptavidin-HRP conjugate. This entire complex (capture antibody/analyte/detector/ Streptavidin-HRP) is immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. The Streptavidin-HRP solution is then added. After further incubation and washing, TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Human MMP-13, matrix metallopeptidase 13, also known as Collagenase 3 is a secreted protein encoded by the gene MMP13. MMP-13 belongs to the matrix metalloproteinase family, which are calcium dependent endopeptidases. MMP-13 degrades type I, II, and III collagen. The MMP protein-13 is expressed as an inactive pro-form composed of an 83-amino acid pro-peptide, an amino acid catalytic domain, and hemopexin-like domain. Dissociation of a zinc ion from the cysteine-switch motif releases the activation-peptide, thus activating the enzyme. MMP-13 is involved in various processes, such as, embryonic development, ossification, wound healing, and tissue remolding.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab221839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of MMP-13 were measured in duplicates, interpolated from the MMP-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, saliva 50%, urine 50%, and RPMI media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-13 concentration was determined to be 32 pg/mL with a range of N.D. – 142 pg/mL.

Protocols

References

ab221839 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Elisa for Osteoarthritis markers on Cartilage explants

Excellent Excellent 5/5 (Ease of Use)
Abreviews
MMP-13 evaluation was performed on media from cartilage explants to determine OA onset. We also tested RPMI and DMEM media to see if it effected out outcome of MMP-13 production in our samples. We treated with IL-1α in ordered to increase synthesis of matrix degrading enzymes including MMP-13. Our first test included" If media had an effect, if matrix degrading enzymes increased, and if the Elisa could detect this.
Assay procedure was performed according to manufacturer instructions. We had to dilute our samples according to our BCA results to what the manufacturer suggested. Readings were performed at 450 nm. And photos show the results
This kit very simple to use if you have a basic knowledge of an Elisa. Our lab will be using more of these.

J Moore

Verified customer

Submitted Jun 06 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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