Human MMP14 ELISA Kit (ab197747)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.137 ng/ml
- Range: 0.391 ng/ml - 25 ng/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MMP14 ELISA Kit
See all MMP14 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 8 2.94% Inter-assay Sample n Mean SD CV% Overall 3 2.52% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.137 ng/ml -
Range
0.391 ng/ml - 25 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 95 93% - 98% Cell culture media 83 79% - 88% Hep Plasma 90 88% - 92% EDTA Plasma 91 90% - 92% Cit plasma 94 91% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam is committed to meeting high quality standards of ethical manufacturing and has decided to discontinue this product because it contains antibodies generated by the ascites method. We are sorry for the inconvenience this may cause. We are currently developing a replacement kit with recombinant monoclonal antibodies.
Human MMP14 ELISA Kit (ab197747) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP14 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MMP14 with 0.137 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
MMP14 is one of six membrane type matrix metalloproteinases, and was the first membrane type MMP discovered. Matrix metalloproteinases are zinc binding enzymes that degrade components of the extracellular matrix, and are involved in tissue remodeling, wound healing, angiogenesis, and tumor invasion. MMP14 may specifically activate progelatinase A and, through pro-gelatinase A activation, trigger tumor cell invasion on the tumor cell surface. MMP14 may also be involved in actin cytoskeleton reorganization by cleaving PTK7, as well as be a positive regulator of cell growth and migration through the activation of MMP15. In mice that were MMP14 gene deficient dysmorphism, arthritis, osteopenia, dwarfism, and fibrosis were observed, which are all conditions related to skeletal and soft tissue modeling.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human MMP14 Capture Antibody 1 x 600µl 10X Human MMP14 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human MMP14 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Function
Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. -
Tissue specificity
Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Membrane. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm. - Information by UniProt
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Alternative names
- Matrix metalloproteinase-14
- Membrane type 1 metalloprotease
- Membrane-type matrix metalloproteinase 1
see all -
Database links
- Entrez Gene: 4323 Human
- Omim: 600754 Human
- SwissProt: P50281 Human
- Unigene: 2399 Human
Associated products
Images
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Other - Human MMP14 ELISA Kit (ab197747)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of MMP14 standard curve prepared in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Example of MMP14 standard curve prepared in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of Human THP-1 cell extract and Human liver homogenate tissue extract within the working range of the assay.The interpolated MMP14 concentration of each lysate dilution is shown.
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Titration of Human serum, plasma, and cell media (+ 10% FBS) spiked with concentrations of recombinant MMP14 protein within the working range of the assay.Background-subtracted data values are graphed.
References (1)
ab197747 has been referenced in 1 publication.
- Schmitt R et al. A potential key mechanism in ascending aortic aneurysm development: Detection of a linear relationship between MMP-14/TIMP-2 ratio and active MMP-2. PLoS One 14:e0212859 (2019). PubMed: 30794673
