Overview

  • Product name
    Human MMP2 ELISA Kit
    See all MMP2 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 3.5 ng/ml
  • Range
    3.3 ng/ml - 800 ng/ml
  • Recovery

    81 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 77.25 69% - 92%
    Serum 81.27 70% - 93%
    Plasma 84.53 72% - 95%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human MMP2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP2 in serum, plasma, and cell culture supernates. (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended as anticoagulants for use in this assay due to their chelating properties).

    This assay employs an antibody specific for Human MMP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    440X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    Biotinylated anti-Human MMP2 (lyophilized) 2 vials
    MMP2 Microplate (12 x 8 wells) 1 unit
    Recombinant Human MMP2 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
    -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
    PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
  • Tissue specificity
    Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
  • Involvement in disease
    Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin-like domains.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
    The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
  • Information by UniProt
  • Alternative names
    • 72 kDa gelatinase
    • 72 kDa type IV collagenase
    • CLG 4
    • CLG 4A
    • CLG4
    • CLG4A
    • Collagenase type IV A
    • EC 3.4.24.24
    • Gelatinase A
    • Gelatinase alpha
    • Gelatinase neutrophil
    • Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
    • Matrix metalloproteinase 2
    • Matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase)
    • Matrix metalloproteinase II
    • Matrix metalloproteinase-2
    • MMP 2
    • MMP II
    • MMP-2
    • MMP2
    • MMP2_HUMAN
    • MONA
    • Neutrophil gelatinase
    • PEX
    • TBE 1
    • TBE-1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • MMP2 measured in biological fluids showing quantity (ng) per mL of tested sample

  • Representative Standard Curve using ab100606.

Protocols

References

This product has been referenced in:
  • Ju Y  et al. Loss of atypical chemokine receptor 4 facilitates C-C motif chemokine ligand 21-mediated tumor growth and invasion in nasopharyngeal carcinoma. Exp Ther Med 17:613-620 (2019). Read more (PubMed: 30651842) »
  • Yu S  et al. Mediating the invasion of smooth muscle cells into a cell-responsive hydrogel under the existence of immune cells. Biomaterials 180:193-205 (2018). Read more (PubMed: 30048909) »
See all 12 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Answer

I can confirm that kits ab100606 and ab100608 recognizes both pro and mature forms of respective proteins.

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MMP2 Human ELISA kit works great

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This kit was very easy to use. I followed the product insert exactly and it worked very well. The results were also reproducible. I would use abcam again for my ELISA or western blotting needs.

Abcam user community

Verified customer

Submitted Jun 18 2013

Answer

Thank you for your inquiry.

I can confirm that these two kits have not been experimentally tested with human cerebrospinal fluid and are therefore not guaranteed to work with this kind of sample.

But we are confident that ab100606 and ab100610 should work with human cerebrospinal fluid. This has to be determined by the customer along with the optimal dilution for human cerebrospinal fluid samples.

I ma sorry that I could not provide you with a more detailed answer on this occasion and wish your customer and you an good start into the new week.

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Answer

Thank you for contacting us.

I now have further information to share with you in regards to the MMP2 ELISA kits, ab100606 and ab100730. The antibodies used within these kits have used the following immunogens:

ab100606 capture antibody: immunogen taken from the N-terminus of human MMP2

detection antibody: immunogentaken from residues 34-660 of human MMP2

ab100730 capture antibody: immunogentaken from residues 34-662ofmouse MMP2

detection antibody: immunogentaken from residues34-660 ofmouse MMP2

The exact epitopes which these antibodies recognise has not been determined and I can therefore not say if they detect the C-terminal or N-terminal part of the protein.

I am sorry that I could not be of more help. If you have any further questions, please do not hesitate to contact us again.

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Answer


Please note this product is unpublished so when you decide to order you need to contact our customer service team for them to put this item in your order.

I hope this info will help. Please let me know if you experience any difficulties.

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Answer

Thank you very much for your kind reply.

I am currently having discussions with developer of this kit regarding the new kit we agreed, sending as a free of charge replacement. This is an extra step for avoiding any further inconvenience due to white precipitates. I will contact you again at the latest tomorrow morning with more updates.

Many thanks for having patience!

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Answer

This product is available but not published on our website. You cannot purchase this using our normal ordering system however I can add this product to your next shipment. Could you confirm how many vials customer would need..6?.
In my last I made an error in calculating the price; the price would be $720 for 6 vials.

The concentration of HRP-streptavidin is not important. The 200ul solution could be used as recommended in protocol. Please note customer have to use new protocol with these new vials, which is available on our website. Each kit protocol have the information on how to use the 200ul vial of HRP-Strep conjugate.

I hope this information will be helpful.

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Question
Answer

Thank you for contacting us. I apologize for the delay.

We are ready to publish this product. The product would be available at US dollar 120 per 200ul. The customer would need 6 vials of product so it will cost them $720 excluding shipping charges. Could you please let us know if customer would like to purchase the product?
Looking forward to hearing from you soon.

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Answer

Thank you for your email. I am sorry for the delay while I was in contact with lab.

The volume of HRP-Streptavidin is very small and therefore would be difficult to see with the naked eye. The HRP-streptavidin volume sent was 8ul and is very concentrated so it is especially important that the vial is spun down before opening to make sure no solution is adhering to the inside walls or cap of the vial to ensure complete recovery. Please have your researchers perform this preparation and they should be able to use the 8 ul HRP. Below are some additional tips for this component:

HRP-Streptavidin – comes as a liquid concentrate. Before withdrawing any liquid from the tube, be sure to centrifuge the vial and pipette up and down to mix thoroughly, as precipitation may form in storage. Once diluted, the HRP-strep must be used up that day. Do not store.

I am also in contact with lab regarding the diluents need to be used for diluting the HRP-Streptavidin vial. I will get back to you with the information soon.

Please note, the expiration date of kit is 6 month from date of purchase including HRP-Streptavidin. Once the HRP streptavidin is reconstituted it should be used same day.

Finally, I can suggest using one kit with the provided HRP-Streptavidin, If in case it does not work then you may buy HRP- streptavidin separately. I will confirm the price if you are interested in buying this.

I hope this information will be helpful. I will contact you with more information soon.

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Answer

Thank you very much for contacting. I apologize for the delay as we were conducting some tests.

We have also seen gloopy things in the Assay buffer which first seen as like fungal growth however when we warmed the buffer at room temperature which is the working temperature of buffer; the precipitates were vanished.

Because the assay diluent contains some proteins and solutes so it would not be uncommon to see cloudiness or floating unsolubilized protein aggregates (especially after storing at low temperatures and/or for a long period of time). We recommend bringing the solution up to room temperature, mixing it thoroughly and diluting to the 1X working concentration which will help clarifying the solution and solubilising any protein aggregates or precipitates. Centrifuging and filtering can also help.

I hope this informaiton will be helpful. Should you have any further question or if these kits does not work properly please do not hesitate to contact me.

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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