Human MMP2 ELISA Kit (ab100606)
- Datasheet
- References (12)
- Protocols
Overview
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Product nameHuman MMP2 ELISA Kit
See all MMP2 kits -
Detection methodColorimetric
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Sample typeCell culture supernatant, Serum, Plasma
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Assay typeSandwich (quantitative)
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Sensitivity< 3.5 ng/ml
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Range3.3 ng/ml - 800 ng/ml
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Recovery
81 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 77.25 69% - 92% Serum 81.27 70% - 93% Plasma 84.53 72% - 95% -
Assay durationMultiple steps standard assay
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Species reactivityReacts with: Human
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Product overview
Abcam’s Human MMP2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP2 in serum, plasma, and cell culture supernates. (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended as anticoagulants for use in this assay due to their chelating properties).
This assay employs an antibody specific for Human MMP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimisation may be required with urine samples.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 440X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated anti-Human MMP2 (lyophilized) 2 vials MMP2 Microplate (12 x 8 wells) 1 unit Recombinant Human MMP2 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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FunctionUbiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. -
Tissue specificityProduced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
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Involvement in diseaseDefects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
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Sequence similaritiesBelongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains. -
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
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Post-translational
modificationsPhosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3. -
Cellular localizationSecreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
- Information by UniProt
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Alternative names
- 72 kDa gelatinase
- 72 kDa type IV collagenase
- CLG 4
see all -
Database links
- Entrez Gene: 4313 Human
- SwissProt: P08253 Human
- Unigene: 513617 Human
Applications
Our Abpromise guarantee covers the use of ab100606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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ab100606 MMP2 Human ELISA kitMMP2 measured in biological fluids showing quantity (ng) per mL of tested sample
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Typical Standard CurveRepresentative Standard Curve using ab100606.
Protocols
References
This product has been referenced in:
- Ju Y et al. Loss of atypical chemokine receptor 4 facilitates C-C motif chemokine ligand 21-mediated tumor growth and invasion in nasopharyngeal carcinoma. Exp Ther Med 17:613-620 (2019). Read more (PubMed: 30651842) »
- Yu S et al. Mediating the invasion of smooth muscle cells into a cell-responsive hydrogel under the existence of immune cells. Biomaterials 180:193-205 (2018). Read more (PubMed: 30048909) »
