Human MMP2 ELISA Kit (ab100606)

I can confirm that kits ab100606 and ab100608 recognizes both pro and mature forms of respective proteins.

Thank you for your inquiry.

I can confirm that these two kits have not been experimentally tested with human cerebrospinal fluid and are therefore not guaranteed to work with this kind of sample.

But we are confident that ab100606 and ab100610 should work with human cerebrospinal fluid. This has to be determined by the customer along with the optimal dilution for human cerebrospinal fluid samples.

I ma sorry that I could not provide you with a more detailed answer on this occasion and wish your customer and you an good start into the new week.

Thank you for contacting us.

I now have further information to share with you in regards to the MMP2 ELISA kits, ab100606 and ab100730. The antibodies used within these kits have used the following immunogens:

ab100606 capture antibody: immunogen taken from the N-terminus of human MMP2

detection antibody: immunogentaken from residues 34-660 of human MMP2

ab100730 capture antibody: immunogentaken from residues 34-662ofmouse MMP2

detection antibody: immunogentaken from residues34-660 ofmouse MMP2

The exact epitopes which these antibodies recognise has not been determined and I can therefore not say if they detect the C-terminal or N-terminal part of the protein.

I am sorry that I could not be of more help. If you have any further questions, please do not hesitate to contact us again.

Please note this product is unpublished so when you decide to order you need to contact our customer service team for them to put this item in your order.

I hope this info will help. Please let me know if you experience any difficulties.

Thank you very much for your kind reply.

I am currently having discussions with developer of this kit regarding the new kit we agreed, sending as a free of charge replacement. This is an extra step for avoiding any further inconvenience due to white precipitates. I will contact you again at the latest tomorrow morning with more updates.

Many thanks for having patience!

This product is available but not published on our website. You cannot purchase this using our normal ordering system however I can add this product to your next shipment. Could you confirm how many vials customer would need..6?.
In my last I made an error in calculating the price; the price would be $720 for 6 vials.

The concentration of HRP-streptavidin is not important. The 200ul solution could be used as recommended in protocol. Please note customer have to use new protocol with these new vials, which is available on our website. Each kit protocol have the information on how to use the 200ul vial of HRP-Strep conjugate.

I hope this information will be helpful.

Thank you for contacting us. I apologize for the delay.

We are ready to publish this product. The product would be available at US dollar 120 per 200ul. The customer would need 6 vials of product so it will cost them $720 excluding shipping charges. Could you please let us know if customer would like to purchase the product?
Looking forward to hearing from you soon.

Thank you for your email. I am sorry for the delay while I was in contact with lab.

The volume of HRP-Streptavidin is very small and therefore would be difficult to see with the naked eye. The HRP-streptavidin volume sent was 8ul and is very concentrated so it is especially important that the vial is spun down before opening to make sure no solution is adhering to the inside walls or cap of the vial to ensure complete recovery. Please have your researchers perform this preparation and they should be able to use the 8 ul HRP. Below are some additional tips for this component:

HRP-Streptavidin – comes as a liquid concentrate. Before withdrawing any liquid from the tube, be sure to centrifuge the vial and pipette up and down to mix thoroughly, as precipitation may form in storage. Once diluted, the HRP-strep must be used up that day. Do not store.

I am also in contact with lab regarding the diluents need to be used for diluting the HRP-Streptavidin vial. I will get back to you with the information soon.

Please note, the expiration date of kit is 6 month from date of purchase including HRP-Streptavidin. Once the HRP streptavidin is reconstituted it should be used same day.

Finally, I can suggest using one kit with the provided HRP-Streptavidin, If in case it does not work then you may buy HRP- streptavidin separately. I will confirm the price if you are interested in buying this.

I hope this information will be helpful. I will contact you with more information soon.

Thank you very much for contacting. I apologize for the delay as we were conducting some tests.

We have also seen gloopy things in the Assay buffer which first seen as like fungal growth however when we warmed the buffer at room temperature which is the working temperature of buffer; the precipitates were vanished.

Because the assay diluent contains some proteins and solutes so it would not be uncommon to see cloudiness or floating unsolubilized protein aggregates (especially after storing at low temperatures and/or for a long period of time). We recommend bringing the solution up to room temperature, mixing it thoroughly and diluting to the 1X working concentration which will help clarifying the solution and solubilising any protein aggregates or precipitates. Centrifuging and filtering can also help.

I hope this informaiton will be helpful. Should you have any further question or if these kits does not work properly please do not hesitate to contact me.