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    human-mmp9-elisa-kitlyophilized-ab100610.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP
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Human MMP9 ELISA Kit (lyophilized) (ab100610)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (2) Submit a question References (33)

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sELISA - MMP9 Human ELISA Kit (ab100610)
  • Typical Standard Curve

Key features and details

  • Sensitivity: 10 pg/ml
  • Range: 8.23 pg/ml - 6000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human MMP9 ELISA Kit (lyophilized)
    See all MMP9 kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    8.23 pg/ml - 6000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.38 84% - 104%
    Serum 96.23 84% - 103%
    Plasma 94.64 83% - 102%
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s MMP9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP9 pro and active forms in serum, plasma (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended), and cell culture supernatants.

    This assay employs an antibody specific for Human MMP9 coated on a 96- well plate. Standards and samples are pipetted into the wells and MMP9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    Biotinylated anti-Human MMP9 2 vials
    MMP9 Microplate (12 x 8 wells) 1 unit
    Recombinant Human MMP9 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Matrix Metalloproteinases
    • MMP
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • MMP
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • ECM enzymes
    • MMPs
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MMPs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • MMPs
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • MMPs
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Other
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    • Processes
  • Function

    May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • Tissue specificity

    Produced by normal alveolar macrophages and granulocytes.
  • Involvement in disease

    Intervertebral disc disease
    Metaphyseal anadysplasia 2
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • Cellular localization

    Secreted, extracellular space, extracellular matrix.
  • Target information above from: UniProt accession P14780 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 82 kDa matrix metalloproteinase-9
    • 92 kDa gelatinase
    • 92 kDa type IV collagenase
    • CLG 4B
    • CLG4B
    • Collagenase Type 4 beta
    • Collagenase type IV 92 KD
    • EC 3.4.24.35
    • Gelatinase 92 KD
    • Gelatinase B
    • Gelatinase beta
    • GelatinaseB
    • GELB
    • Macrophage gelatinase
    • MANDP2
    • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
    • Matrix Metalloproteinase 9
    • MMP 9
    • MMP-9
    • MMP9
    • MMP9_HUMAN
    • Type V collagenase
    see all
  • Database links

    • Entrez Gene: 4318 Human
    • Omim: 120361 Human
    • SwissProt: P14780 Human
    • Unigene: 297413 Human

    Images

    • sELISA - MMP9 Human ELISA Kit (ab100610)
      sELISA - MMP9 Human ELISA Kit (ab100610)
      MMP9 measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).
    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab100610.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (33)

    Publishing research using ab100610? Please let us know so that we can cite the reference in this datasheet.

    ab100610 has been referenced in 33 publications.

    • Shi G & Zhang Z Rap2B promotes the proliferation and migration of human glioma cells via activation of the ERK pathway. Oncol Lett 21:314 (2021). PubMed: 33692846
    • Ozuna H  et al. The Hunger Games: Aggregatibacter actinomycetemcomitans Exploits Human Neutrophils As an Epinephrine Source for Survival. Front Immunol 12:707096 (2021). PubMed: 34456916
    • Grassi ES  et al. Hypoxia-induced release, nuclear translocation, and signaling activity of a DLK1 intracellular fragment in glioma. Oncogene 39:4028-4044 (2020). PubMed: 32205867
    • Sun P  et al. RAB9A Plays an Oncogenic Role in Human Liver Cancer Cells. Biomed Res Int 2020:5691671 (2020). PubMed: 32420351
    • Yang X  et al. Inhibition of ITGB1 enhance the anti-tumor effect of cetuximab in colorectal cancer cell. Medicine (Baltimore) 99:e20944 (2020). PubMed: 32629699
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    MMP9 quantification in Normal Human Dermal Fibroblasts supernatants

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    75000 cells/well were seeded in a 6-well plate for 96 hours in serum-free medium with an induction with TNF-alpha alone or a mix of TNF-alpha and TGF-beta. Medium were collected and centrifuged at 2000 rpm for 10 minutes, then supernatants were transferred to 1.5 ml tubes and kept at -80°C until use.
    The concentrations of MMP9 were quantified with commercially available ELISA kit (Abcam, catalog number ab100610) according to the manufacturer´s protocol without any dilution.
    All samples were assayed in triplicates using a microplate reader and values were reported as ng/mL. The mean absorbance for each set of duplicate standards and samples was calculated. The standard curve was plotted in a log10-log10 scale.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 16 2017

    MMP9 quantification in uterine aspirate samples

    Good Good 4/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    Uterine aspirates were collected by aspiration with a Cornier Pipelle (Eurogine Ref. 03040200) in the office of the clinician or in the operating room prior to surgery and transferred to 1.5 ml microtubes. Phosphate buffer saline was added in a 1:1 (v/v) ratio and centrifuged at 2,500 rcf for 20 min in order to separate the soluble fraction (supernatant) from the solid fraction (pellet). The supernatants were kept at -80°C until use.
    The concentrations of MMP9 in the soluble fraction of uterine aspirates were quantified with commercially available ELISA kit (Abcam, catalog number ab100610) according to the manufacturer´s protocol . As no results are available regarding the levels of these proteins in uterine aspirates, samples were diluted using five serial dilutions: no dilution, 1:10, 1:100, 1:1000, 1:10000.
    The same amount of total protein from 6 uterine aspirate samples was loaded in each well. All samples were assayed in duplicates using a microplate reader and values were reported as ng/mL. The mean absorbance for each set of duplicate standards and samples was calculated. The standard curve was plotted in a log10-log10 scale (see figure 1A). The CV (%) between the duplicates of the samples ranged from 0-13% (average of 4%). Concentration of MMP9 of uterine aspirates ranged from 1 to 664 ng/u (see figure 1B).
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 19 2016

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