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    human-mmp9-matched-antibody-pair-kit-ab213741.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP
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Recombinant

Human MMP9 Matched Antibody Pair Kit (ab213741)

  • Datasheet
  • SDS
  • Protocol Booklet
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Human MMP9 standard curve.
  • Sandwich ELISA - Human MMP9 Matched Antibody Pair Kit (ab213741)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Human
  • Range: 15.6 pg/ml - 1000 pg/ml

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Overview

  • Product name

    Human MMP9 Matched Antibody Pair Kit
    See all MMP9 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Sensitivity

    3.77 pg/ml
  • Range

    15.6 pg/ml - 1000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human MMP9 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human MMP9.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 5 x 96 tests
    Human MMP9 Capture Antibody 1 x 50µg
    Human MMP9 Detector Antibody 1 x 12.5µg
    Human MMP9 Lyophilized Protein 1 vial
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Matrix Metalloproteinases
    • MMP
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • MMP
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • ECM enzymes
    • MMPs
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MMPs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • MMPs
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • MMPs
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Other
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    • Processes
  • Function

    May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • Tissue specificity

    Produced by normal alveolar macrophages and granulocytes.
  • Involvement in disease

    Intervertebral disc disease
    Metaphyseal anadysplasia 2
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • Cellular localization

    Secreted, extracellular space, extracellular matrix.
  • Target information above from: UniProt accession P14780 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 82 kDa matrix metalloproteinase-9
    • 92 kDa gelatinase
    • 92 kDa type IV collagenase
    • CLG 4B
    • CLG4B
    • Collagenase Type 4 beta
    • Collagenase type IV 92 KD
    • EC 3.4.24.35
    • Gelatinase 92 KD
    • Gelatinase B
    • Gelatinase beta
    • GelatinaseB
    • GELB
    • Macrophage gelatinase
    • MANDP2
    • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
    • Matrix Metalloproteinase 9
    • MMP 9
    • MMP-9
    • MMP9
    • MMP9_HUMAN
    • Type V collagenase
    see all
  • Database links

    • Entrez Gene: 4318 Human
    • Omim: 120361 Human
    • SwissProt: P14780 Human
    • Unigene: 297413 Human

    Associated products

    • Alternative Versions

      • Human MMP9 Antibody Pair - BSA and Azide free (ab241787)
    • Related Products

      • ELISA Accessory Pack (ab210905)

    Images

    • Human MMP9 standard curve.
      Human MMP9 standard curve.

      Standard calibration curve. Background subtracted values are graphed. Data provided for demonstration purposes only.

    • Sandwich ELISA - Human MMP9 Matched Antibody Pair Kit (ab213741)
      Sandwich ELISA - Human MMP9 Matched Antibody Pair Kit (ab213741)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab213741? Please let us know so that we can cite the reference in this datasheet.

    ab213741 has not yet been referenced specifically in any publications.

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