Overview

  • Product name

    Human MS2 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 0.22ng/ml 0.013 5.7%
    2 16 1.47ng/ml 0.09 6.1%
    3 16 2.34ng/ml 0.185 7.9%
    Inter-assay
    Sample n Mean SD CV%
    1 24 0.27ng/ml 0.018 6.8%
    2 24 1.56ng/ml 0.115 7.4%
    3 24 2.58ng/ml 0.214 8.3%
  • Sample type

    Cell culture supernatant, Serum
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human MS2 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213750) is designed for the quantitative measurement of Human MS2 in cell culture supernatants and serum.


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for MS2 has been pre-coated onto 96-well plates. Standards (NSO, M1-P497) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MS2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MS2 amount of sample captured in plate.

  • Notes

    MS2 (also known as ADAM8), a disintegrin and metalloproteinase domain-containing protein 8 is an enzyme that in humans is encoded by the MS2 /ADAM8 gene. MS2 is localized to chromosome 10q26.3. This gene encodes a member of the ADAM (a disintegrin and metalloproteinase domain) family. Members of the ADAM family, such as MS2, are cell surface proteases involved in remodeling of extracellular matrix, cell migration, and processing of membrane-bound signaling molecules. They are characterized by disintegrin and metalloprotease domains that confer adhesive properties and proteolytic activities, respectively. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab213750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human ADAM8 ELISA Kit (ab213750) Standard Curve.

Protocols

References

ab213750 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab213750.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up