Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Mumps virus IgM ELISA Kit
Intra-assay Sample n Mean SD CV% Pos.Serum 8 4.6% Inter-assay Sample n Mean SD CV% Pos.Serum 15 5.5%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Mumps virus IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Mumps virus in Human serum and plasma.
A 96-well plate has been precoated with Mumps virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Mumps virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mumps virus IgM sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 unit IgM Sample Diluent white cap 1 x 100ml Mumps virus (IgM) Coated Microplate (12 x 8 wells) 12 breakapart 8-well snap-off strips 1 unit Mumps virus anti-IgM HRP Conjugate black cap 1 x 20ml Mumps virus IgM Cut-off Control green cap 1 x 3ml Mumps virus IgM Negative Control blue cap 1 x 2ml Mumps virus IgM Positive Control red cap 1 x 2ml Stop Solution red cap 1 x 15ml TMB Substrate Solution Yellow cap 1 unit
Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
ab108753 has not yet been referenced specifically in any publications.