Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Mycoplasma pneumoniae IgG ELISA Kit
Intra-assay Sample n Mean SD CV% Pos. Serum 20 5.3% Pos. Serum 24 9% Inter-assay Sample n Mean SD CV% Pos. Serum 12 3.4% Pos. Serum 12 6.9%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Mycoplasma pneumoniae IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Mycoplasma pneumoniae in Human serum and plasma.
A 96-well plate has been precoated with Mycoplasma pneumoniae antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Mycoplasma pneumoniae-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mycoplasma pneumoniae IgG sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml IgG Sample Diluent white cap 1 x 100ml Mycoplasma pneumoniae (IgG) Coated Microplate (12 x 8 wells) 12 breakapart 8-well snap-off strips 1 unit Mycoplasma pneumoniae anti-IgG HRP Conjugate black cap 1 x 20ml Mycoplasma pneumoniae IgG Cut-off Control green cap 1 x 3ml Mycoplasma pneumoniae IgG Negative Control blue cap 1 x 2ml Mycoplasma pneumoniae IgG Positive Control red cap 1 x 2ml Stop Solution red cap 1 x 15ml TMB Substrate Solution Yellow cap 1 x 15ml
ab108755 has not yet been referenced specifically in any publications.