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Immunology Immunoglobulins Heavy Chain IgM
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Human Anti-Mycoplasma pneumoniae IgM ELISA Kit (ab108756)

  • Datasheet
  • SDS
  • Protocol Booklet
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Serologic ELISA assay principle

    Key features and details

    • Sample type: Cit plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Indirect
    • Reacts with: Human

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    Protein
    Product image
    Native Human IgG FC fragment protein (ab90285)

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    Overview

    • Product name

      Human Anti-Mycoplasma pneumoniae IgM ELISA Kit
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Pos. Serum 31 2.9%
      Pos. Serum 22 9.2%
      Inter-assay
      Sample n Mean SD CV%
      Pos. Serum 12 4.8%
      Pos. Serum 12 7.8%
    • Sample type

      Serum, Hep Plasma, Cit plasma
    • Assay type

      Indirect
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s anti-Mycoplasma pneumoniae IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Mycoplasma pneumoniae in Human serum and plasma.

       

      A 96-well plate has been precoated with Mycoplasma pneumoniae antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Mycoplasma pneumoniae-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mycoplasma pneumoniae IgM sample captured in plate.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      20X Washing Solution White cap 1 x 50ml
      Cover foil 1 unit
      IgM Sample Diluent Green, White cap 1 x 100ml
      Mycoplasma pneumoniae (IgM) Coated Microplate (12 x 8 wells) 12 x 8 Well Strips 1 unit
      Mycoplasma pneumoniae anti-IgM HRP Conjugate Red, Black cap 1 x 20ml
      Mycoplasma pneumoniae IgM Cut-off Control Yellow, Green cap 1 x 3ml
      Mycoplasma pneumoniae IgM Negative Control Yellow, Blue cap 1 x 2ml
      Mycoplasma pneumoniae IgM Positive Control Yellow, Red cap 1 x 2ml
      Stop Solution red cap 1 x 15ml
      Strip holder 1 unit
      TMB Substrate Solution Yellow cap 1 x 15ml
    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgM
      • Microbiology
      • Organism
      • Bacteria
      • Gram negative Bacteria
      • Mycoplasma
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Pathogen specific immunoglobulins ELISA kits
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Infectious disease ELISA kits

    Associated products

      Images

      • Serologic ELISA assay principle
        Serologic ELISA assay principle
        Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab108756? Please let us know so that we can cite the reference in this datasheet.

      ab108756 has not yet been referenced specifically in any publications.

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