Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Mycoplasma pneumoniae IgM ELISA Kit
Intra-assay Sample n Mean SD CV% Pos. Serum 31 2.9% Pos. Serum 22 9.2% Inter-assay Sample n Mean SD CV% Pos. Serum 12 4.8% Pos. Serum 12 7.8%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Mycoplasma pneumoniae IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Mycoplasma pneumoniae in Human serum and plasma.
A 96-well plate has been precoated with Mycoplasma pneumoniae antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Mycoplasma pneumoniae-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mycoplasma pneumoniae IgM sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit IgM Sample Diluent Green, White cap 1 x 100ml Mycoplasma pneumoniae (IgM) Coated Microplate (12 x 8 wells) 12 x 8 Well Strips 1 unit Mycoplasma pneumoniae anti-IgM HRP Conjugate Red, Black cap 1 x 20ml Mycoplasma pneumoniae IgM Cut-off Control Yellow, Green cap 1 x 3ml Mycoplasma pneumoniae IgM Negative Control Yellow, Blue cap 1 x 2ml Mycoplasma pneumoniae IgM Positive Control Yellow, Red cap 1 x 2ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
ab108756 has not yet been referenced specifically in any publications.