Human Anti-Mycoplasma pneumoniae IgM ELISA Kit (ab108756)
- Datasheet
- References
- Protocols
Overview
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Product name
Human Anti-Mycoplasma pneumoniae IgM ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Pos. Serum 31 2.9% Pos. Serum 22 9.2% Inter-assay Sample n Mean SD CV% Pos. Serum 12 4.8% Pos. Serum 12 7.8% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Indirect -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s anti-Mycoplasma pneumoniae IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Mycoplasma pneumoniae in Human serum and plasma.
A 96-well plate has been precoated with Mycoplasma pneumoniae antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Mycoplasma pneumoniae-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mycoplasma pneumoniae IgM sample captured in plate.
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Tested applications
Suitable for: Indirect ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit IgM Sample Diluent Green, White cap 1 x 100ml Mycoplasma pneumoniae (IgM) Coated Microplate (12 x 8 wells) 12 x 8 Well Strips 1 unit Mycoplasma pneumoniae anti-IgM HRP Conjugate Red, Black cap 1 x 20ml Mycoplasma pneumoniae IgM Cut-off Control Yellow, Green cap 1 x 3ml Mycoplasma pneumoniae IgM Negative Control Yellow, Blue cap 1 x 2ml Mycoplasma pneumoniae IgM Positive Control Yellow, Red cap 1 x 2ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
Applications
Our Abpromise guarantee covers the use of ab108756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Indirect ELISA | Use at an assay dependent dilution. |
Protocols
References
ab108756 has not yet been referenced specifically in any publications.
