Human MyD88 ELISA Kit (ab171341)

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Typical standard curve

    Key features and details

    • Sensitivity: 10 pg/ml
    • Range: 156 pg/ml - 10000 pg/ml
    • Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    Overview

    • Product name

      Human MyD88 ELISA Kit
      See all MyD88 kits

    • Detection method

      Colorimetric

    • Sample type

      Cell culture supernatant, Cell Lysate, Hep Plasma, EDTA Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      < 10 pg/ml

    • Range

      156 pg/ml - 10000 pg/ml

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Human

    • Product overview

      Abcam’s Human MyD88 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human MyD88 in cell culture supernatants.

      A MyD88 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for MyD88 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human MyD88 amount of sample captured in plate.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      ABC Diluent Buffer Blue Cap 1 x 12ml
      Antibody Diluent Buffer Green Cap 1 x 12ml
      Anti-Human MyD88 Antibody Microplate (12 x 8 wells) 1 x 96 tests
      Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
      Biotinylated anti-Human MyD88 antibody 1 x 100µl
      Lyophilized recombinant Human MyD88 standard 2 x 10ng
      Plate Seal 1 x 4 units
      Sample Diluent Buffer Green Cap 1 x 30ml
      TMB Color Developing Agent Amber Bottle 1 x 10ml
      TMB Stop Solution Yellow Cap 1 x 10ml

    • Research areas

    • Function

      Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.

    • Tissue specificity

      Ubiquitous.

    • Involvement in disease

      Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.

    • Sequence similarities

      Contains 1 death domain.
      Contains 1 TIR domain.

    • Domain

      The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Alternative names

      • Mutant myeloid differentiation primary response 88
      • MYD 88
      • Myd88
      • MYD88_HUMAN
      • MYD88D
      • Myeloid differentiation marker 88
      • Myeloid differentiation primary response 88
      • Myeloid differentiation primary response gene
      • Myeloid differentiation primary response gene (88)
      • Myeloid differentiation primary response gene 88
      • Myeloid differentiation primary response protein MyD88
      • OTTHUMP00000161718
      • OTTHUMP00000208595
      • OTTHUMP00000209058
      • OTTHUMP00000209059
      • OTTHUMP00000209060
      see all

    • Database links

      Images

      • Typical standard curve
        Typical standard curve

        Representative standard curve using ab171341

      References (0)

      Publishing research using ab171341? Please let us know so that we can cite the reference in this datasheet.

      ab171341 has not yet been referenced specifically in any publications.

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