Human MyD88 ELISA Kit (ab171341)
Overview
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Product name
Human MyD88 ELISA Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
156 pg/ml - 10000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human MyD88 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human MyD88 in cell culture supernatants.
A MyD88 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for MyD88 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human MyD88 amount of sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human MyD88 Antibody Microplate (12 x 8 wells) 1 x 96 tests Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-Human MyD88 antibody 1 x 130µl Lyophilized recombinant Human MyD88 standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. -
Tissue specificity
Ubiquitous. -
Involvement in disease
MYD88 deficiency (MYD88D) [MIM:612260]: Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age. Note=The disease is caused by mutations affecting the gene represented in this entry. -
Sequence similarities
Contains 1 death domain.
Contains 1 TIR domain. -
Domain
The intermediate domain (ID) is required for the phosphorylation and activation of IRAK. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- Myd88
- MYD88_HUMAN
- MYD88D
see all -
Database links
- Entrez Gene: 4615 Human
- Omim: 602170 Human
- SwissProt: Q99836 Human
- Unigene: 82116 Human
Images
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Typical standard curveRepresentative standard curve using ab171341
References
ab171341 has not yet been referenced specifically in any publications.
