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Immunology Innate Immunity Macrophage / Inflamm.
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Human Myeloperoxidase ELISA Kit (MPO) (ab119605)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1)Q&A (2)References (10)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 10 pg/ml
    • Range: 312 pg/ml - 20000 pg/ml
    • Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Saliva, Serum, Tissue Homogenate, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Overview

    • Product name

      Human Myeloperoxidase ELISA Kit (MPO)
      See all Myeloperoxidase kits
    • Detection method

      Colorimetric
    • Sample type

      Cell culture supernatant, Saliva, Urine, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 10 pg/ml
    • Range

      312 pg/ml - 20000 pg/ml
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s Human Myeloperoxidase (MPO) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Myeloperoxidase in cell culture supernatants, cell lysate, tissue homogenates, serum, plasma (heparin, EDTA), saliva and urine.


      A Myeloperoxidase specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Myeloperoxidase is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Myeloperoxidase amount of sample captured in plate.


      Get results in 90 minutes with Human Myeloperoxidase ELISA Kit (ab195212) from our SimpleStep ELISA® range.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      ABC Diluent Buffer Blue Cap 1 x 12ml
      Antibody Diluent Buffer Green Cap 1 x 12ml
      Anti-Human Myeloperoxidase antibody Microplate (12 x 8 wells) 1 unit
      Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
      Biotinylated anti-Human Myeloperoxidase antibody 1 x 130µl
      Lyophilized recombinant Human Myeloperoxidase standard 2 x 20ng
      Plate Seal 4 units
      Sample Diluent Buffer Green Cap 1 x 30ml
      TMB Color Developing Agent Amber Bottle 1 x 10ml
      TMB Stop Solution Yellow Cap 1 x 10ml
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Cardiovascular
      • Blood
      • Other
      • Cardiovascular
      • Vasculature
      • Vasculature Markers
      • Arterial
      • Cardiovascular
      • Vasculature
      • Vasculature Markers
      • Pulmonary
      • Cancer
      • Cancer Metabolism
      • Cellular metabolic process
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      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Antioxidants
    • Function

      Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
    • Involvement in disease

      Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
    • Sequence similarities

      Belongs to the peroxidase family. XPO subfamily.
    • Cellular localization

      Lysosome.
    • Target information above from: UniProt accession P05164 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • 84 kDa myeloperoxidase
      • 89 kDa myeloperoxidase
      • EC 1.11.1.7
      • EC1.11.2.2
      • fj80f04
      • MPO
      • mpx
      • myeloid-specific peroxidase
      • Myeloperoxidase
      • Myeloperoxidase heavy chain
      • Myeloperoxidase light chain
      • PERM_HUMAN
      • wu:fj80f04
      see all
    • Database links

      • Entrez Gene: 4353 Human
      • Omim: 606989 Human
      • SwissProt: P05164 Human
      • Unigene: 458272 Human

      Associated products

        Images

        • Typical Standard Curve
          Typical Standard Curve

          Representative Standard Curve using ab119605.

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • SDS download

        • Datasheet download

          Download

        References (10)

        Publishing research using ab119605? Please let us know so that we can cite the reference in this datasheet.

        ab119605 has been referenced in 10 publications.

        • Isaacs AW  et al. C-Reactive Protein Is Elevated Only in High Creatine Kinase Responders to Muscle Damaging Exercise. Front Physiol 10:86 (2019). PubMed: 30804809
        • Koupenova M  et al. The role of platelets in mediating a response to human influenza infection. Nat Commun 10:1780 (2019). PubMed: 30992428
        • Han G  et al. Annexin A1-mediated inhibition of inflammatory cytokines may facilitate the resolution of inflammation in acute radiation-induced lung injury. Oncol Lett 18:321-329 (2019). PubMed: 31289503
        • Garcia-Martinez I  et al. A leukocyte activation test identifies food items which induce release of DNA by innate immune peripheral blood leucocytes. Nutr Metab (Lond) 15:26 (2018). PubMed: 29651299
        • Petersen KS  et al. Grape polyphenols corrects ageing-related detriments in neutrophil functionality via modulation of specific molecular targets. Inflammopharmacology 26:1349-1358 (2018). PubMed: 29951779
        • Egorov AI  et al. Vegetated land cover near residence is associated with reduced allostatic load and improved biomarkers of neuroendocrine, metabolic and immune functions. Environ Res 158:508-521 (2017). PubMed: 28709033
        • Aldabbous L  et al. Neutrophil Extracellular Traps Promote Angiogenesis: Evidence From Vascular Pathology in Pulmonary Hypertension. Arterioscler Thromb Vasc Biol 36:2078-87 (2016). PubMed: 27470511
        • Li L  et al. Phenol-soluble modulin a4 mediates Staphylococcus aureus-associated vascular leakage by stimulating heparin-binding protein release from neutrophils. Sci Rep 6:29373 (2016). PubMed: 27383625
        • Chu Y  et al. Myeloperoxidase is increased in human cerebral aneurysms and increases formation and rupture of cerebral aneurysms in mice. Stroke 46:1651-6 (2015). PubMed: 25922506
        • Zhou C  et al. Decreased progranulin levels in patients and rats with subarachnoid hemorrhage: a potential role in inhibiting inflammation by suppressing neutrophil recruitment. J Neuroinflammation 12:200 (2015). Rat . PubMed: 26527034

        Customer reviews and Q&As

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        1-3 of 3 Abreviews or Q&A

        MPO quantification in uterine aspirate samples

        Good Good 4/5 (Ease of Use)
        Abreviews
        Abreviews
        abreview image
        Uterine aspirates were collected by aspiration with a Cornier Pipelle (Eurogine Ref. 03040200) in the office of the clinician or in the operating room prior to surgery and transferred to 1.5 ml microtubes. Phosphate buffer saline was added in a 1:1 (v/v) ratio and centrifuged at 2,500 rcf for 20 min in order to separate the soluble fraction (supernatant) from the solid fraction (pellet). The supernatants were kept at -80°C until use.
        The concentrations of MPO in the soluble fraction of uterine aspirates were quantified with commercially available ELISA kit (Abcam, catalog number ab119605) according to the manufacturer´s protocol. As no results are available regarding the levels of these proteins in uterine aspirates, samples were diluted using five serial dilutions: 1:2, 1:10, 1:50, 1:300, 1:1500.
        The same amount of total protein from 8 uterine aspirates was loaded in each well. All samples were assayed in duplicates using a microplate reader and values were reported as ng/mL. The mean absorbance for each set of duplicate standards and samples was calculated. The standard curve was plotted in a log10-log10 scale (see figure 1A). The CV (%) between the duplicates of the samples ranged from 4-13% (average of 7%). Concentration of MMP9 of uterine aspirates ranged from 2 to 1053 ng/ul (see figure 1B).

        Abcam user community

        Verified customer

        Submitted Apr 18 2016

        Question

        has the antibody in this kit been tested for cross-reactivity with other molecules such as VPO? Or can you compare the immunogen sequence for potential cross-reactivity?

        Read More

        Abcam community

        Verified customer

        Asked on Apr 17 2013

        Answer

        Thank you for contacting us. Cross-reactivity with other molecules such as VPO has not been tested, however the homology between the immunogen of the antibody in the kit and human VPO is 41%, much to low for cross-reactivity to occurs. Typically around 85% there is cross-reactivity.
        I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

        Read More

        Abcam Scientific Support

        Answered on Apr 17 2013

        Question

        Dear technical team,

        Our customer is looking for MPO ELISA kit for mouse tissue sample.
        There's only one kit in abcam's catalog.
        https://www.abcam.com/MPO-Human-ELISA-KIt-ab119605.html

        It stated Human kit but, I would like to ask if there's any possibility to work on mouse sample.
        Check this please.

        Best regards,

        Read More

        Abcam community

        Verified customer

        Asked on Jul 17 2012

        Answer

        Thank you for your enquiry.

        I can confirm that this kit (ab119605: MPO Human ELISA Kit) has been tested for cross-reactivity with human samples. It doesn't work on mouse samples.

        If you need any further assistance in the future, please do not hesitate to contact me.

        Read More

        Abcam Scientific Support

        Answered on Jul 17 2012

        Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
        For licensing inquiries, please contact partnerships@abcam.com

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