Human Myeloperoxidase ELISA Kit (MPO) (ab119605)

Reviews (1)Q&A (2)References (10)
Typical Standard Curve

    Key features and details

    • Sensitivity: 10 pg/ml
    • Range: 312 pg/ml - 20000 pg/ml
    • Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Saliva, Serum, Tissue Homogenate, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    Overview

    • Product name

      Human Myeloperoxidase ELISA Kit (MPO)
      See all Myeloperoxidase kits

    • Detection method

      Colorimetric

    • Sample type

      Cell culture supernatant, Saliva, Urine, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      < 10 pg/ml

    • Range

      312 pg/ml - 20000 pg/ml

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Human

    • Product overview

      Abcam’s Human Myeloperoxidase (MPO) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Myeloperoxidase in cell culture supernatants, cell lysate, tissue homogenates, serum, plasma (heparin, EDTA), saliva and urine.


      A Myeloperoxidase specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Myeloperoxidase is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Myeloperoxidase amount of sample captured in plate.


      Get results in 90 minutes with Human Myeloperoxidase ELISA Kit (ab195212) from our SimpleStep ELISA® range.

    • Platform

      Microplate

    Properties

    Associated products

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab119605.

      References (10)

      Publishing research using ab119605? Please let us know so that we can cite the reference in this datasheet.

      ab119605 has been referenced in 10 publications.

      • Isaacs AW  et al. C-Reactive Protein Is Elevated Only in High Creatine Kinase Responders to Muscle Damaging Exercise. Front Physiol 10:86 (2019). PubMed: 30804809
      • Koupenova M  et al. The role of platelets in mediating a response to human influenza infection. Nat Commun 10:1780 (2019). PubMed: 30992428
      • Han G  et al. Annexin A1-mediated inhibition of inflammatory cytokines may facilitate the resolution of inflammation in acute radiation-induced lung injury. Oncol Lett 18:321-329 (2019). PubMed: 31289503
      • Garcia-Martinez I  et al. A leukocyte activation test identifies food items which induce release of DNA by innate immune peripheral blood leucocytes. Nutr Metab (Lond) 15:26 (2018). PubMed: 29651299
      • Petersen KS  et al. Grape polyphenols corrects ageing-related detriments in neutrophil functionality via modulation of specific molecular targets. Inflammopharmacology 26:1349-1358 (2018). PubMed: 29951779
      • Egorov AI  et al. Vegetated land cover near residence is associated with reduced allostatic load and improved biomarkers of neuroendocrine, metabolic and immune functions. Environ Res 158:508-521 (2017). PubMed: 28709033
      • Aldabbous L  et al. Neutrophil Extracellular Traps Promote Angiogenesis: Evidence From Vascular Pathology in Pulmonary Hypertension. Arterioscler Thromb Vasc Biol 36:2078-87 (2016). PubMed: 27470511
      • Li L  et al. Phenol-soluble modulin a4 mediates Staphylococcus aureus-associated vascular leakage by stimulating heparin-binding protein release from neutrophils. Sci Rep 6:29373 (2016). PubMed: 27383625
      • Chu Y  et al. Myeloperoxidase is increased in human cerebral aneurysms and increases formation and rupture of cerebral aneurysms in mice. Stroke 46:1651-6 (2015). PubMed: 25922506
      • Zhou C  et al. Decreased progranulin levels in patients and rats with subarachnoid hemorrhage: a potential role in inhibiting inflammation by suppressing neutrophil recruitment. J Neuroinflammation 12:200 (2015). Rat . PubMed: 26527034

      Customer reviews and Q&As

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      MPO quantification in uterine aspirate samples

       Good Good 4/5 (Ease of Use)
      Abreviews
      abreview image
      Uterine aspirates were collected by aspiration with a Cornier Pipelle (Eurogine Ref. 03040200) in the office of the clinician or in the operating room prior to surgery and transferred to 1.5 ml microtubes. Phosphate buffer saline was added in a 1:1 (v/v) ratio and centrifuged at 2,500 rcf for 20 min in order to separate the soluble fraction (supernatant) from the solid fraction (pellet). The supernatants were kept at -80°C until use.
      The concentrations of MPO in the soluble fraction of uterine aspirates were quantified with commercially available ELISA kit (Abcam, catalog number ab119605) according to the manufacturer´s protocol. As no results are available regarding the levels of these proteins in uterine aspirates, samples were diluted using five serial dilutions: 1:2, 1:10, 1:50, 1:300, 1:1500.
      The same amount of total protein from 8 uterine aspirates was loaded in each well. All samples were assayed in duplicates using a microplate reader and values were reported as ng/mL. The mean absorbance for each set of duplicate standards and samples was calculated. The standard curve was plotted in a log10-log10 scale (see figure 1A). The CV (%) between the duplicates of the samples ranged from 4-13% (average of 7%). Concentration of MMP9 of uterine aspirates ranged from 2 to 1053 ng/ul (see figure 1B).

      Abcam user community

      Verified customer

      Submitted Apr 18 2016

      Question

      has the antibody in this kit been tested for cross-reactivity with other molecules such as VPO? Or can you compare the immunogen sequence for potential cross-reactivity?

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      Answer

      Thank you for contacting us. Cross-reactivity with other molecules such as VPO has not been tested, however the homology between the immunogen of the antibody in the kit and human VPO is 41%, much to low for cross-reactivity to occurs. Typically around 85% there is cross-reactivity.
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Question

      Dear technical team,

      Our customer is looking for MPO ELISA kit for mouse tissue sample.
      There's only one kit in abcam's catalog.
      https://www.abcam.com/MPO-Human-ELISA-KIt-ab119605.html

      It stated Human kit but, I would like to ask if there's any possibility to work on mouse sample.
      Check this please.

      Best regards,

      Read More
      Answer

      Thank you for your enquiry.

      I can confirm that this kit (ab119605: MPO Human ELISA Kit) has been tested for cross-reactivity with human samples. It doesn't work on mouse samples.

      If you need any further assistance in the future, please do not hesitate to contact me.

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