Human Myeloperoxidase ELISA Kit (MPO) (ab119605)

Reviews (1)Q&A (2)References (5)

Overview

  • Product name
    Human Myeloperoxidase ELISA Kit (MPO)
    See all Myeloperoxidase kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Saliva, Urine, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    312 pg/ml - 20000 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human Myeloperoxidase (MPO) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Myeloperoxidase in cell culture supernatants, cell lysate, tissue homogenates, serum, plasma (heparin, EDTA), saliva and urine.


    A Myeloperoxidase specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Myeloperoxidase is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Myeloperoxidase amount of sample captured in plate.


    Get results in 90 minutes with Human Myeloperoxidase ELISA Kit (ab195212) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab119605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab119605.

Protocols

References

This product has been referenced in:
  • Garcia-Martinez I  et al. A leukocyte activation test identifies food items which induce release of DNA by innate immune peripheral blood leucocytes. Nutr Metab (Lond) 15:26 (2018). Read more (PubMed: 29651299) »
  • Petersen KS  et al. Grape polyphenols corrects ageing-related detriments in neutrophil functionality via modulation of specific molecular targets. Inflammopharmacology 26:1349-1358 (2018). Read more (PubMed: 29951779) »
See all 5 Publications for this product

Publishing research using ab119605? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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MPO quantification in uterine aspirate samples

 Good Good 4/5 (Ease of Use)
Abreviews
abreview image
Uterine aspirates were collected by aspiration with a Cornier Pipelle (Eurogine Ref. 03040200) in the office of the clinician or in the operating room prior to surgery and transferred to 1.5 ml microtubes. Phosphate buffer saline was added in a 1:1 (v/v) ratio and centrifuged at 2,500 rcf for 20 min in order to separate the soluble fraction (supernatant) from the solid fraction (pellet). The supernatants were kept at -80°C until use.
The concentrations of MPO in the soluble fraction of uterine aspirates were quantified with commercially available ELISA kit (Abcam, catalog number ab119605) according to the manufacturer´s protocol. As no results are available regarding the levels of these proteins in uterine aspirates, samples were diluted using five serial dilutions: 1:2, 1:10, 1:50, 1:300, 1:1500.
The same amount of total protein from 8 uterine aspirates was loaded in each well. All samples were assayed in duplicates using a microplate reader and values were reported as ng/mL. The mean absorbance for each set of duplicate standards and samples was calculated. The standard curve was plotted in a log10-log10 scale (see figure 1A). The CV (%) between the duplicates of the samples ranged from 4-13% (average of 7%). Concentration of MMP9 of uterine aspirates ranged from 2 to 1053 ng/ul (see figure 1B).

Abcam user community

Verified customer

Submitted Apr 18 2016

Question

has the antibody in this kit been tested for cross-reactivity with other molecules such as VPO? Or can you compare the immunogen sequence for potential cross-reactivity?

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Answer

Thank you for contacting us. Cross-reactivity with other molecules such as VPO has not been tested, however the homology between the immunogen of the antibody in the kit and human VPO is 41%, much to low for cross-reactivity to occurs. Typically around 85% there is cross-reactivity.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Dear technical team,

Our customer is looking for MPO ELISA kit for mouse tissue sample.
There's only one kit in abcam's catalog.
https://www.abcam.com/MPO-Human-ELISA-KIt-ab119605.html

It stated Human kit but, I would like to ask if there's any possibility to work on mouse sample.
Check this please.

Best regards,

Read More
Answer

Thank you for your enquiry.

I can confirm that this kit (ab119605: MPO Human ELISA Kit) has been tested for cross-reactivity with human samples. It doesn't work on mouse samples.

If you need any further assistance in the future, please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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