Overview

  • Product name

    Human Myeloperoxidase ELISA Kit (MPO)
    See all Myeloperoxidase kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Human MPO 24 9.6ng/ml = 1.1%
    Human MPO 24 2ng/ml = 1.3%
    Human MPO 24 3.6ng/ml = 3.6%
    Human MPO 24 10.3ng/ml = 1.6%
    Human MPO 24 2ng/ml = 1.9%
    Human MPO 24 1ng/ml = 3.6%
    Inter-assay
    Sample n Mean SD CV%
    Human MPO 9.9ng/ml = 3.5%
    Human MPO 2ng/ml = 3.5%
    Human MPO 0.98ng/ml = 3.1%
    Human MPO 10ng/ml = 1.9%
    Human MPO 1.9ng/ml = 3.6%
    Human MPO 0.95ng/ml = 7.2%
  • Sample type

    Urine, Tissue Culture Media, Sputum Supernatant, Nasal Lavage Supernatant, Lithium Heparin Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    >= 0.019 ng/ml
  • Range

    0.195 ng/ml - 12.5 ng/ml
  • Recovery

    = 98.6 %

    Sample specific recovery
    Sample type Average % Range
    Urine = 93.2 % - %
    Tissue Culture Media = 95 % - %
    Heparin Plasma = 105 % - %
    Sputum Supernatant = 101 % - %
    Nasal Lavage Supernatant = 98.8 % - %

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Human Myeloperoxidase (MPO) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Myeloperoxidase in Tissue Culture Media, Sputum Supernatant, Nasal Lavage Supernatant, Lithium Heparin Plasma, and Urine.


    A Myeloperoxidase mouse monoclonal antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, incubated and then washed. A Myeloperoxidase polyclonal antibody is then added, incubated and washed. An HRP conjugated anti-IgG antibody is then added, incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.


    Get results in 90 minutes with Human Myeloperoxidase ELISA Kit (ab195212) from our SimpleStep ELISA® range.

  • Notes

    Compound Cross Reactivity
    Human Myeloperoxidase 100%
    Human Erythropoietin 1.10%
    Bovine Lactoperoxidase 0.40%
    Human Elastase 0.20%
    Human Lactoferrin 0.20%
    Sheep COX-2 0.10%
    Human Thyroid Peroxidase < 0.1%
    Human Eosinophil-derived Neurotoxin < 0.1%
  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab136943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab136943 Assay Buffer 13.

  • Representative Standard Curve using ab136943. Assay Buffer 31.

Protocols

References

ab136943 has not yet been referenced specifically in any publications.

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