Overview

  • Product name

    Human Myoglobin ELISA Kit
    See all Myoglobin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    homogenate 5 2%
    Inter-assay
    Sample n Mean SD CV%
    homogenate 3 2%
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    270 pg/ml
  • Range

    1.25 ng/ml - 80 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 75 67% - 81%
    Serum 103 83% - 115%
    Tissue Extracts 111 105% - 118%
    Cell culture media 101 81% - 113%
    Hep Plasma 89 86% - 92%
    EDTA Plasma 98 90% - 110%
    Cit plasma 99 82% - 108%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    As of November 2, 2018, Human Myoglobin SimpleStep ELISA® kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency when quantifying Myoglobin protein in human serum, plasma, urine, cell culture supernatant, cell and tissue extracts.


    Human Myoglobin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA. Quantitate human myoglobin with 270 pg/ml sensitivity.

    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips

    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

  • Notes

    Myoglobin, a heme protein is found in both cardiac and skeletal muscle and functions as a reserve supply of oxygen by facilitating the movement of oxygen within muscles. Damage to either type of muscle following conditions such as trauma, ischemia, and diseases that cause myopathy, is associated with the release of myoglobin into serum. Specifically, following cardiac necrosis associated with myocardial infarction (MI), myoglobin is one of the first markers to rise above normal levels. Myoglobin levels increase measurably above baseline within 2-4 hours post-infarct, peaking at 9-12 hours, and returning to baseline within 24-36 hours.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab171580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The Myoglobin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The Myoglobin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Myoglobin were measured in duplicates, interpolated from the Myoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (heparin) 25%, plasma (citrate) 25%, plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 15.8 ng/mL in serum, 15.6 ng/mL in plasma (citrate), 8.3 ng/mL in plasma (EDTA) and 3.4 ng/mL in plasma (heparin).

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). 5 of 10 donors were below the detectable dose.  The mean Myoglobin concentration was determined to be 5.4 ng/mL with a range of ND – 9.8 ng/mL.

  • The concentrations of Myoglobin were measured in duplicate and interpolated from the Myoglobin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 361 ng/mL in Human Heart Homogenate.

  • The concentrations of Myoglobin were measured in duplicates, interpolated from the Myoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25% and urine 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Protocols

References

ab171580 has not yet been referenced specifically in any publications.

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