Product nameHuman Myoglobin ELISA Kit
See all Myoglobin kits
Intra-assay Sample n Mean SD CV% Heart Homog 9 4.5% Inter-assay Sample n Mean SD CV% Heart Homog 3 7.8%
Sample typeCell culture supernatant, Serum, Plasma, Cell Lysate, Cell culture media, Tissue Lysate
Assay typeSandwich (quantitative)
Range0.63 ng/ml - 40 ng/ml
Sample specific recovery Sample type Average % Range Cell Lysate 86 82% - 89% 143B Cell Lysate 115 108% - 119%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Abcam’s Myoglobin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Myoglobin protein in human serum, plasma, cell culture supernatants, cell extracts and tissue extracts.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Myoglobin, a heme protein is found in both cardiac and skeletal muscle and functions as a reserve supply of oxygen by facilitating the movement of oxygen within muscles. Damage to either type of muscle following conditions such as trauma, ischemia, and diseases that cause myopathy, is associated with the release of myoglobin into serum. Specifically, following cardiac necrosis associated with myocardial infarction (MI), myoglobin is one of the first markers to rise above normal levels. Myoglobin levels increase measurably above baseline within 2-4 hours post-infarct, peaking at 9-12 hours, and returning to baseline within 24-36 hours.
Tested applicationsSuitable for: Sandwich ELISAmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Myoglobin Capture Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml ab221824 - Antibody Diluent 4BI 1 x 6ml Myoglobin Detector Antibody (Lyophilized) 2 x 600ng Myoglobin Human Lyophilized Purified Protein 2 x 10ng Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 x 96 tests Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
FunctionServes as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Sequence similaritiesBelongs to the globin family.
- Information by UniProt
Our Abpromise guarantee covers the use of ab171580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab171580 has not yet been referenced specifically in any publications.