Overview

  • Product name
    Human Myoglobin ELISA Kit
    See all Myoglobin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    homogenate 5 2%
    Inter-assay
    Sample n Mean SD CV%
    homogenate 3 2%
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Cell culture extracts, Tissue Extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    270 pg/ml
  • Range
    1.25 ng/ml - 500 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 75 67% - 81%
    Serum 103 83% - 115%
    Tissue Extracts 111 105% - 118%
    Cell culture media 101 81% - 113%
    Heparin Plasma 89 86% - 92%
    EDTA Plasma 98 90% - 110%
    Citrate Plasma 99 82% - 108%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Human Myoglobin SimpleStep ELISA® kit (ab171580) has been redeveloped with new capture and detector antibodies. While this does not affect the specificity of the results, it may result in increased sensitivity and will improve the consistency in the performance of the kit. 


    Myoglobin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Myoglobin protein in human serum, plasma, urine, cell culture supernatant, cell and tissue extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Myoglobin, a heme protein is found in both cardiac and skeletal muscle and functions as a reserve supply of oxygen by facilitating the movement of oxygen within muscles. Damage to either type of muscle following conditions such as trauma, ischemia, and diseases that cause myopathy, is associated with the release of myoglobin into serum. Specifically, following cardiac necrosis associated with myocardial infarction (MI), myoglobin is one of the first markers to rise above normal levels. Myoglobin levels increase measurably above baseline within 2-4 hours post-infarct, peaking at 9-12 hours, and returning to baseline within 24-36 hours.


    Sensitivity:


    Samples diluted in 1X Cell Extraction Buffer PTR  – 1200 pg/mL


    Samples diluted in Sample Diluent NS – 270 pg/mL


     

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab171580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • The Myoglobin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The Myoglobin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Myoglobin were measured in duplicates, interpolated from the Myoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (heparin) 25%, plasma (citrate) 25%, plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 15.8 ng/mL in serum, 15.6 ng/mL in plasma (citrate), 8.3 ng/mL in plasma (EDTA) and 3.4 ng/mL in plasma (heparin).

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). 5 of 10 donors were below the detectable dose.  The mean Myoglobin concentration was determined to be 5.4 ng/mL with a range of ND – 9.8 ng/mL.

  • The concentrations of Myoglobin were measured in duplicate and interpolated from the Myoglobin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 361 ng/mL in Human Heart Homogenate.

  • The concentrations of Myoglobin were measured in duplicates, interpolated from the Myoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25% and urine 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Protocols

References

ab171580 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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