Key features and details
- One-wash 90 minute protocol
- Sensitivity: 270 pg/ml
- Range: 1.25 ng/ml - 80 ng/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Myoglobin ELISA Kit
See all Myoglobin kits
Intra-assay Sample n Mean SD CV% homogenate 5 2% Inter-assay Sample n Mean SD CV% homogenate 3 2%
Sample typeCell culture supernatant, Urine, Serum, Plasma, Cell culture extracts, Tissue Extracts
Assay typeSandwich (quantitative)
Range1.25 ng/ml - 80 ng/ml
Sample specific recovery Sample type Average % Range Urine 75 67% - 81% Serum 103 83% - 115% Tissue Extracts 111 105% - 118% Cell culture media 101 81% - 113% Hep Plasma 89 86% - 92% EDTA Plasma 98 90% - 110% Cit plasma 99 82% - 108%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Human Myoglobin ELISA kit (ab171580) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Myoglobin protein in human serum, plasma, urine, cell culture supernatant, cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human myoglobin with 270 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
Myoglobin, a heme protein is found in both cardiac and skeletal muscle and functions as a reserve supply of oxygen by facilitating the movement of oxygen within muscles. Damage to either type of muscle following conditions such as trauma, ischemia, and diseases that cause myopathy, is associated with the release of myoglobin into serum. Specifically, following cardiac necrosis associated with myocardial infarction (MI), myoglobin is one of the first markers to rise above normal levels. Myoglobin levels increase measurably above baseline within 2-4 hours post-infarct, peaking at 9-12 hours, and returning to baseline within 24-36 hours.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml 10X Human Myoglobin Capture Antibody 1 x 600µl 10X Human Myoglobin Detector Antibody 1 x 600µl Human Myoglobin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 x 96 tests Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
FunctionServes as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Sequence similaritiesBelongs to the globin family.
- Information by UniProt
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
The Myoglobin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of Myoglobin were measured in duplicates, interpolated from the Myoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (heparin) 25%, plasma (citrate) 25%, plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 15.8 ng/mL in serum, 15.6 ng/mL in plasma (citrate), 8.3 ng/mL in plasma (EDTA) and 3.4 ng/mL in plasma (heparin).
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). 5 of 10 donors were below the detectable dose. The mean Myoglobin concentration was determined to be 5.4 ng/mL with a range of ND – 9.8 ng/mL.
The concentrations of Myoglobin were measured in duplicates, interpolated from the Myoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25% and urine 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
To learn more about the advantages of recombinant antibodies see here.
ab171580 has not yet been referenced specifically in any publications.