Human NBL1 ELISA Kit (ab99997)
Key features and details
- Sensitivity: 35 pg/ml
- Range: 27.43 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human NBL1 ELISA Kit
See all NBL1 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 35 pg/ml -
Range
27.43 pg/ml - 20000 pg/ml -
Recovery
95 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 111.3 107% - 122% Serum 100.1 91% - 109% Plasma 81.82 72% - 90% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s NBL1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NBL1 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human NBL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and NBL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human NBL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NBL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm..
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human NBL1 2 vials NBL1 Microplate (12 strips x 8 wells) 1 unit Recombinant Human NBL1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
- Information by UniProt
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Alternative names
- D1S1733E
- DAN
- DAN domain family member 1
see all -
Database links
- Entrez Gene: 100532736 Human
- Entrez Gene: 4681 Human
- Omim: 600613 Human
- SwissProt: P41271 Human
- Unigene: 466662 Human
- Unigene: 654502 Human
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab99997 has not yet been referenced specifically in any publications.