Human NCAM1 ELISA Kit (CD56) (ab119587)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 78 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human NCAM1 ELISA Kit (CD56)
See all NCAM1 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
78 pg/ml - 5000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human NCAM1 (CD56) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human NCAM1 (CD56) in cell culture supernatants, serum and plasma (heparin, EDTA).
A NCAM1 (CD56) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for NCAM1 (CD56) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human NCAM1 (CD56) amount of sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-Human NCAM1 antibody Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti- Human NCAM1 antibody 1 x 100µl 1 x 100µl Lyophilized recombinant Human NCAM1 standard 2 x 10ng 2 x 10ng Plate Seal 4 units 4 units Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
Research areas
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Function
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. -
Sequence similarities
Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- antigen MSK39 identified by monoclonal antibody 5.1H11
- antigen recognized by monoclonal antibody 5.1H11
- CD56
see all -
Database links
- Entrez Gene: 4684 Human
- Omim: 116930 Human
- SwissProt: P13591 Human
- Unigene: 503878 Human
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab119587 has been referenced in 1 publication.
- Ziegler S et al. CD56 Is a Pathogen Recognition Receptor on Human Natural Killer Cells. Sci Rep 7:6138 (2017). ELISA ; Human . PubMed: 28733594