Overview

  • Product name

    Human NCAM1 ELISA Kit (CD56)
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    78 pg/ml - 5000 pg/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Human NCAM1 (CD56) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human NCAM1 (CD56) in cell culture supernatants, serum and plasma (heparin, EDTA).

    A NCAM1 (CD56) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for NCAM1 (CD56) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human NCAM1 (CD56) amount of sample captured in plate.

  • Tested applications

    Suitable for: ELISA, Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab119587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab119587.

Protocols

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your interest in the CD44 and CD56 ELISA kits, ab45912 and ab119587. Neither has been tested for reactivity with mouse or rat samples. We are unable to assess the probability of cross-reaction by BLAST, as the locations of the epitopes that the antibodies recognize are unknown. In general, without data showing otherwise, it is best to assume there will be some cross-reaction.

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Abreview for CD56 Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Serum (Heparin plasma)
Specification: Heparin plasma
Amount of Sample: 1 uL (x100 dilution) µg
Type of Kit Used: ELISA
Positive control: Kit standards
Did you use the Abcam Kit protocol?: Yes

Abcam user community

Verified customer

Submitted Sep 12 2012

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