Key features and details
- Sensitivity: 10 pg/ml
- Range: 78 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman NCAM1 ELISA Kit (CD56)
Sample typeCell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
Assay typeSandwich (quantitative)
Sensitivity< 10 pg/ml
Range78 pg/ml - 5000 pg/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Human NCAM1 (CD56) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human NCAM1 (CD56) in cell culture supernatants, serum and plasma (heparin, EDTA).
A NCAM1 (CD56) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for NCAM1 (CD56) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human NCAM1 (CD56) amount of sample captured in plate.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human NCAM1 antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti- Human NCAM1 antibody 1 x 130µl Lyophilized recombinant Human NCAM1 standard 2 x 10ng Plate Seal 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml
FunctionThis protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Sequence similaritiesContains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
ab119587 has been referenced in 1 publication.
- Ziegler S et al. CD56 Is a Pathogen Recognition Receptor on Human Natural Killer Cells. Sci Rep 7:6138 (2017). ELISA ; Human . PubMed: 28733594