Human Neuro Antibody Array II (Membrane, 30 Targets) (ab211063)

Overview

  • Product name

    Human Neuro Antibody Array II (Membrane, 30 Targets)
  • Sample type

    Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Human Neuro Antibody Array II (ab211063) for use with cell culture media, serum, plasma, cell and tissue lysates and other liquid samples.


    Targets: Adiponectin, BDNF, beta-NGF, CNTF, CRP, Eotaxin-1, Eotaxin-2, Eotaxin-3, Fas, IL-4, GDNF, GM-CSF, IFN-gamma, IL-10, IL-18, IL-1 alpha, IL-1 beta, IL-6, IL-8, LIF, MCP-1, MIP-1 beta, MMP-2, MMP-3, S100B, TARC, TGF beta, TIMP-1, TNF alpha, VEGF-A.

  • Notes

    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1 mL of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

    Learn more about cytokine arrays and other membrane antibody arrays

  • Tested applications

    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 2 Membrane 4 Membrane 8 Membrane
    1,000X HRP-Streptavidin Buffer 1 x 50µl 1 x 50µl 1 x 50µl
    20X Wash Buffer I 1 x 10ml 1 x 10ml 1 x 20ml
    20X Wash Buffer II 1 x 10ml 1 x 10ml 1 x 20ml
    2X Cell Lysis Buffer 1 x 10ml 1 x 10ml 1 x 16ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit
    Antibody Arrays 2 units 4 units 8 units
    Biotinylated Antibody Cocktail 1 vial 2 vials 4 vials
    1X Blocking Buffer 1 x 25ml 1 x 25ml 2 x 25ml
    Detection Buffer C 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml
    Detection Buffer D 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml
    Plastic sheets 1 unit 1 unit 1 unit

Applications

Our Abpromise guarantee covers the use of ab211063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

Protocols

References

ab211063 has not yet been referenced specifically in any publications.

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