Overview

  • Product name

    Human Neuropilin-1 ELISA Kit
    See all Neuropilin 1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Human Serum 3 1.9%
    Inter-assay
    Sample n Mean SD CV%
    Human Serum 5 3%
  • Sample type

    Milk, Urine, Serum, Cell culture extracts, Tissue Extracts, Tissue Culture Media, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    7 pg/ml
  • Range

    37.5 pg/ml - 4800 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Milk 89 84% - 97%
    Urine 83 81% - 85%
    Serum 110 107% - 113%
    Cell culture extracts 95 86% - 105%
    Tissue Extracts 100 94% - 104%
    Heparin Plasma 95 91% - 97%
    EDTA Plasma 108 106% - 110%
    Citrate Plasma 114 111% - 119%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Predicted to work with: CowDoes not react with: Mouse, Rat, Non human primates
  • Product overview

    Neuropilin-1 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Neuropilin-1 protein in human serum, plasma, milk, urine, cell culture supernatants, and cell and tissue extract samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    The membrane-bound isoform 1 of Neuropilin-1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. The soluble isoform 2 of Neuropilin-1 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. Its expression has an adverse effect on blood vessel number and integrity.


    Sensitivity:
    Samples diluted in Sample Diluent NS - 7 pg/mL
    Samples diluted in 1X Cell Extraction Buffer PTR - 17.3 pg/mL

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    10X Human Neuropilin-1 Capture Antibody 1 x 600µl
    10X Human Neuropilin-1 Detector Antibody 1 x 600µl
    Human Neuropilin-1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.
    The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
  • Tissue specificity

    The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
  • Sequence similarities

    Belongs to the neuropilin family.
    Contains 2 CUB domains.
    Contains 2 F5/8 type C domains.
    Contains 1 MAM domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names

    • A5 protein
    • BDCA4
    • BLOOD DENDRITIC CELL ANTIGEN 4
    • CD304
    • Neuropilin-1
    • Neuropilin1
    • NP1
    • NPN1
    • NRP
    • NRP 1
    • NRP1
    • NRP1_HUMAN
    • transmembrane receptor
    • Vascular endothelial cell growth factor 165 receptor
    • VEGF165R
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab227901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between human Neuropilin-1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Neuropilin-1 were measured in duplicates, interpolated from the Neuropilin-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (citrate) 1:100, plasma (heparin) 1:100 and plasma (EDTA) 1:100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuropilin-1 concentration was determined to be 330.1 ng/mL in neat serum, 249.9 ng/mL in neat plasma (citrate), 232.5 ng/mL in neat plasma (heparin), and 268.1 ng/mL in neat plasma (EDTA).

  • The concentrations of Neuropilin-1 were measured in duplicates, interpolated from the Neuropilin-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 1:50 and urine 1:8. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuropilin-1 concentration was determined to be 24.31 ng/mL in neat breast milk and 10.12 ng/mL in neat urine.

  • The concentrations of Neuropilin-1 were measured in duplicate and interpolated from the Neuropilin-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuropilin-1 concentration was determined to be 2.982 ng/mL in HT-29 cell extract and 2.732 ng/mL in human heart tissue extract.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuropilin-1 concentration was determined to be 702.5 ng/mL with a range of 533.6 – 937.3 ng/mL.

Protocols

References

ab227901 has not yet been referenced specifically in any publications.

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