Human Neurotrophin 3 ELISA Kit (ab100615)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameHuman Neurotrophin 3 ELISA Kit
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Detection methodColorimetric
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Sample typeCell culture supernatant, Serum, Plasma
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Assay typeSandwich (quantitative)
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Sensitivity< 4 pg/ml
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Range4.12 pg/ml - 3000 pg/ml
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Recovery
96 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 97.25 87% - 105% Serum 95 85% - 103% Plasma 94.45 84% - 105% -
Assay durationMultiple steps standard assay
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Species reactivityReacts with: Human
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Product overview
Abcam’s Neurotrophin 3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Neurotrophin 3 in serum, plasma, and cell culture supernatants. (Human Neurotrophin 3 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human Neurotrophin 3 coated on a 96-well plate. Standards and samples are pipetted into the wells and Neurotrophin 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Neurotrophin 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Neurotrophin 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human Neurotrophin 3 (lyophilized) 2 vials Neurotrophin 3 Microplate (12 x 8 wells) 1 unit Recombinant Human Neurotrophin 3 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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RelevanceNeurotrophin 3 (NT3) is part of the family of neurotrophic factors that control survival and differentiation of mammalian neurons. NT3 is closely related to both nerve growth factor and brain derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and affect development of neurons in the embryo when it is expressed in human placenta. NT3 deficient mice generated by gene targeting display severe movement defects of the limbs.
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Alternative names
- AI316846
- AI835689
- HDNF
see all -
Database links
- Entrez Gene: 4908 Human
- Omim: 162660 Human
- SwissProt: P20783 Human
Applications
Our Abpromise guarantee covers the use of ab100615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Sandwich ELISA - Neurotrophin 3 Human ELISA kit (ab100615)NT-3 measured in biologicals showing quantity (pg) per mL tested sample
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Typical Standard CurveRepresentative Standard Curve using ab100615.
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Typical Standard CurveRepresentative Standard Curve using ab100615.
Protocols
References
This product has been referenced in:
- Stewart AN et al. Transplantation of Mesenchymal Stem Cells that Overexpress NT-3 Produce Motor Improvements without Axonal Regeneration following Complete Spinal Cord Transections in Rats. Brain Res N/A:N/A (2018). ELISA . Read more (PubMed: 29883625) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"