Key features and details
- Sensitivity: 4 pg/ml
- Range: 4.12 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Neurotrophin 3 ELISA Kit
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 4 pg/ml
Range4.12 pg/ml - 3000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 97.25 87% - 105% Serum 95 85% - 103% Plasma 94.45 84% - 105%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam is committed to meeting high quality standards of ethical manufacturing and will discontinue this product when inventory is depleted because it contains antibodies generated by the ascites method. We are sorry for the inconvenience this may cause.
Abcam’s Neurotrophin 3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Neurotrophin 3 in serum, plasma, and cell culture supernatants. (Human Neurotrophin 3 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human Neurotrophin 3 coated on a 96-well plate. Standards and samples are pipetted into the wells and Neurotrophin 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Neurotrophin 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Neurotrophin 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human Neurotrophin 3 (lyophilized) 2 vials Neurotrophin 3 Microplate (12 x 8 wells) 1 unit Recombinant Human Neurotrophin 3 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceNeurotrophin 3 (NT3) is part of the family of neurotrophic factors that control survival and differentiation of mammalian neurons. NT3 is closely related to both nerve growth factor and brain derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and affect development of neurons in the embryo when it is expressed in human placenta. NT3 deficient mice generated by gene targeting display severe movement defects of the limbs.
ab100615 has been referenced in 1 publication.
- Stewart AN et al. Transplantation of Mesenchymal Stem Cells that Overexpress NT-3 Produce Motor Improvements without Axonal Regeneration following Complete Spinal Cord Transections in Rats. Brain Res N/A:N/A (2018). ELISA . PubMed: 29883625