Overview

  • Product name
    Human NGF R ELISA Kit (CD271)
    See all NGFR kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Intra-assay < 10%
    Inter-assay
    Sample n Mean SD CV%
    Inter-assay < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 80 pg/ml
  • Range
    0.082 ng/ml - 20 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 86.88 79% - 95%
    Serum 82.75 75% - 93%
    Plasma 84.17 76% - 93%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s NGF R (CD271) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human NGF R (CD271) in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human NGF R coated on a 96-well plate. Standards and samples are pipetted into the wells and NGF R present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human NGF R antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NGF R bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent C 1 x 30ml
    Biotinylated anti-Human NGF R 2 vials
    NGF R Microplate (12 x 8 wells) 1 unit
    Recombinant Human NGF R Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver.
  • Cellular localization
    Membrane; Single-pass type I membrane protein.
  • Alternative names
    • CD271
    • Gp80-LNGFR
    • Low affinity neurotrophin receptor p75NTR
    • Low-affinity nerve growth factor receptor
    • Nerve growth factor receptor
    • NGF receptor
    • NGFR
    • p75 ICD
    • p75(NTR)
    • p75NTR
    • TNFR superfamily member 16
    • TNFRSF16
    • Tumor necrosis factor receptor superfamily member 16
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab155436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab155436

Protocols

References

This product has been referenced in:
  • Jiang H  et al. The serum protein levels of the tPA-BDNF pathway are implicated in depression and antidepressant treatment. Transl Psychiatry 7:e1079 (2017). ELISA . Read more (PubMed: 28375203) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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