Key features and details
- Sensitivity: 20 pg/ml
- Range: 0.029 ng/ml - 7 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Notch-1 ELISA Kit (TAN-1)
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 20 pg/ml
Range0.029 ng/ml - 7 ng/ml
> 86 %
Sample specific recovery Sample type Average % Range Serum = 96.06 88% - 102% Plasma = 93.78 86% - 102% Cell culture media = 103.1 93% - 113%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Notch-1 (TAN-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human Notch-1 (TAN-1) in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Notch-1(TAN-1) coated on a 96-well plate. Standards and samples are pipetted into the wells and Notch-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated Anti-Human Notch-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Notch-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
This kit can detect extracellular domain of NOTCH1.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human Notch-1 2 vials Notch-1 Microplate (12 x 8 wells) 1 unit Recombinant Human Notch-1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
ab155437 has not yet been referenced specifically in any publications.