Key features and details
- Sensitivity: 2 pg/ml
- Range: 2 pg/ml - 30000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman NOV ELISA Kit (Nephroblastoma Overexpressed)
See all NOV kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range2 pg/ml - 30000 pg/ml
Sample specific recovery Sample type Average % Range Serum 109.5 102% - 118% Plasma 99.29 93% - 106% Cell culture media 130.4 118% - 139%
Assay durationOne step assay
Species reactivityReacts with: Human
Abcam’s NOV (Nephroblastoma Overexpressed) Human ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human NOV in serum, plasma, and cell culture supernatant.
This assay employs an antibody specific for human NOV coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures NOV present in the samples. The wells are washed and biotinylated anti-human NOV antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of NOV bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated Human NOV detection antibody (lyophilized) 2 vials Human NOV Standards (lyophilized) 2 vials Pre-coated Human NOV Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionImmediate-early protein likely to play a role in cell growth regulation.
Tissue specificityExpressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type.
Sequence similaritiesBelongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain.
- Information by UniProt
- CCN family member 3
- IGF-binding protein 9
ab193710 has been referenced in 1 publication.
- Li H et al. The Predictive Role of Tenascin-C and Cellular Communication Network Factor 3 (CCN3) in Post Hepatectomy Liver Failure in a Rat Model and 50 Patients Following Partial Hepatectomy. Med Sci Monit 25:6755-6766 (2019). PubMed: 31494663