Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
![Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)](https://www.abcam.com/ps/products/191/ab191181/Images/ab191181-336808-anti-human-nuclear-antigen-antibody-235-1-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)")
Key features and details
- Mouse monoclonal [235-1] to Human Nuclear Antigen
- Suitable for: IHC-Fr
- Reacts with: Human, Non human primates
- Isotype: IgG1
Overview
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Product name
Anti-Human Nuclear Antigen antibody [235-1]
See all Human Nuclear Antigen primary antibodies -
Description
Mouse monoclonal [235-1] to Human Nuclear Antigen -
Host species
Mouse -
Specificity
ab191181 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in frozen tissue sections. It can be used with paraformaldehyde-fixed frozen tissue or cell preparations. -
Tested applications
Suitable for: IHC-Frmore details -
Species reactivity
Reacts with: Human, Non human primates
Does not react with: Mouse, Rat, Chicken -
Immunogen
Tissue, cells or virus corresponding to Human Nuclear Antigen. (Immunogen: Nuclei of Human myeloid leukemia biopsy cells).
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Positive control
- IHC-Fr: Human tonsil tissue.
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General notes
ab190710 is recommended for IHC-P with paraffin embedded sections.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
235-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab191181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use a concentration of 0.5 - 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Alternative names
- HNA antibody
- HuNu antibody
Images
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Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)Rockne et al PLoS One. 2018 Aug 2;13(8):e0199967. doi: 10.1371/journal.pone.0199967. eCollection 2018. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Characterization of LM-NSC008 cells.
Visualization of LM-NSC008 cells in naïve non-tumor bearing mouse brain 6 and 9 months post administration. (A) Immunohistochemistry (IHC) staining of LM-NSC008 cells (stably expressing eGFP/Ffluc) with anti-HNA antibodies (NSC injection site, white arrows indicate the LM-NSC008 cells, scale bar 100 μm). (1a-1d) Insets show LM-NSC008 cells co-expressing HNA and eGFP proteins (inset scale bars [1a-1f] 2 μm).
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![Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)](https://www.abcam.com/ps/products/191/ab191181/Images/ab191181-280636-anti-human-nuclear-antigen-antibody-235-1-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)Cold acetone-fixed frozen human tonsil tissue stained for Human Nuclear Antigen with ab191181 at 0.5 µg/ml (30 mins, RT) in immunohistochemical analysis. Visualised using a HRP-Polymer detection system.
Protocols
References (23)
ab191181 has been referenced in 23 publications.
- Deng R et al. Haemin pre-treatment augments the cardiac protection of mesenchymal stem cells by inhibiting mitochondrial fission and improving survival. J Cell Mol Med 24:431-440 (2020). PubMed: 31660694
- Shi Y et al. Vascularized human cortical organoids (vOrganoids) model cortical development in vivo. PLoS Biol 18:e3000705 (2020). PubMed: 32401820
- Horowitz LF et al. Multiplexed drug testing of tumor slices using a microfluidic platform. NPJ Precis Oncol 4:12 (2020). PubMed: 32435696
- Zhang Y et al. Macrophage migration inhibitory factor rejuvenates aged human mesenchymal stem cells and improves myocardial repair. Aging (Albany NY) 11:12641-12660 (2019). PubMed: 31881006
- Xiao M et al. Synergistic effects of stromal cell-derived factor-1a and bone morphogenetic protein-2 treatment on odontogenic differentiation of human stem cells from apical papilla cultured in the VitroGel 3D system. Cell Tissue Res 378:207-220 (2019). PubMed: 31152245
