Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
Mouse monoclonal Human Nuclear Antigen antibody [235-1]. Validated in IP, IHC, ICC, Flow Cyt, ICC/IF and tested in Human, Non human primates. Cited in 9 publication(s).
- Datasheet
- References (16)
- Protocols
Overview
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Product name
Anti-Human Nuclear Antigen antibody [235-1]
See all Human Nuclear Antigen primary antibodies -
Description
Mouse monoclonal [235-1] to Human Nuclear Antigen -
Host species
Mouse -
Specificity
ab191181 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in frozen tissue sections. It can be used with paraformaldehyde-fixed frozen tissue or cell preparations. -
Tested applications
Suitable for: Flow Cyt, ICC, IP, ICC/IF, IHC-Frmore details -
Species reactivity
Reacts with: Human, Non human primates
Does not react with: Mouse, Rat, Chicken -
Immunogen
Tissue, cells or virus corresponding to Human Nuclear Antigen. (Immunogen: Nuclei of Human myeloid leukemia biopsy cells).
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Positive control
- IHC-Fr: Human tonsil tissue.
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General notes
ab190710 is recommended for IHC-P with paraffin embedded sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
235-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab191181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.5-1µg for 106 cells. | |
| ICC | Use a concentration of 0.25 - 0.5 µg/ml. Use Acetone fixed cells. |
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| IP | Use at 1-2 µg/mg of lysate. | |
| ICC/IF | Use a concentration of 0.5 - 1 µg/ml. | |
| IHC-Fr | Use a concentration of 0.5 - 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Alternative names
- HNA antibody
- HuNu antibody
Images
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![Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)](https://www.abcam.com/ps/products/191/ab191181/Images/ab191181-336808-anti-human-nuclear-antigen-antibody-235-1-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)Rockne et al PLoS One. 2018 Aug 2;13(8):e0199967. doi: 10.1371/journal.pone.0199967. eCollection 2018. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Characterization of LM-NSC008 cells.
Visualization of LM-NSC008 cells in naïve non-tumor bearing mouse brain 6 and 9 months post administration. (A) Immunohistochemistry (IHC) staining of LM-NSC008 cells (stably expressing eGFP/Ffluc) with anti-HNA antibodies (NSC injection site, white arrows indicate the LM-NSC008 cells, scale bar 100 μm). (1a-1d) Insets show LM-NSC008 cells co-expressing HNA and eGFP proteins (inset scale bars [1a-1f] 2 μm).
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![Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)](https://www.abcam.com/ps/products/191/ab191181/Images/ab191181-336807-anti-human-nuclear-antigen-antibody-235-1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)Verified customerTop panels: PFA fixed, Triton X-100 permeabilized human cell line labeling Human Nuclear Antigen using ab191181 at 1 µg/ml (red) in ICC/IF. Nuclear probe is visualized in blue.
Bottom panels: Rabbit sample, RAB-9 skin fibroblast cells (No cross reactivity).
For more details please see abreview.
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![Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)](https://www.abcam.com/ps/products/191/ab191181/Images/ab191181-280636-anti-human-nuclear-antigen-antibody-235-1-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)Cold acetone-fixed frozen human tonsil tissue stained for Human Nuclear Antigen with ab191181 at 0.5 µg/ml (30 mins, RT) in immunohistochemical analysis. Visualised using a HRP-Polymer detection system.
Protocols
References
This product has been referenced in:
- Zou T et al. Organoid-derived C-Kit+/SSEA4- human retinal progenitor cells promote a protective retinal microenvironment during transplantation in rodents. Nat Commun 10:1205 (2019). Read more (PubMed: 30872578) »
- Ham SW et al. Korean Red ginseng extract inhibits glioblastoma propagation by blocking the Wnt signaling pathway. J Ethnopharmacol 236:393-400 (2019). Read more (PubMed: 30878548) »
