Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
- Datasheet
- Specific References (8)
- Protocols
Overview
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Product nameAnti-Human Nuclear Antigen antibody [235-1]
See all Human Nuclear Antigen primary antibodies -
DescriptionMouse Monoclonal [235-1] to Human Nuclear Antigen
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Host speciesMouse
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Specificityab191181 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in frozen tissue sections. It can be used with paraformaldehyde-fixed frozen tissue or cell preparations.
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Tested applicationsSuitable for: Flow Cyt, ICC, IP, ICC/IF, IHC-Frmore details
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Species reactivityReacts with: Human, Non human primates
Does not react with: Mouse, Rat, Chicken -
Immunogen
Tissue, cells or virus corresponding to Human Human Nuclear Antigen . (Immunogen: Nuclei of Human myeloid leukemia biopsy cells).
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Positive control
- Human tonsil.
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General notes
ab190710 is recommned for IHC-P with paraffin embedded sections.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone number235-1
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Related Products
Applications
Our Abpromise guarantee covers the use of ab191181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.5-1µg for 106 cells. | |
| ICC | Use a concentration of 0.25 - 0.5 µg/ml. Use Acetone fixed cells. |
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| IP | Use at 1-2 µg/mg of lysate. | |
| ICC/IF | Use a concentration of 0.5 - 1 µg/ml. | |
| IHC-Fr | Use a concentration of 0.5 - 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Alternative names
- HNA antibody
- HuNu antibody
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)](https://www.abcam.com/ps/products/191/ab191181/Images/ab191181-280941-Human-Nu-Ag-2351HeLaPhalloidin488HeLa3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody was used at 1ug/ml for 60 minutes at room temp. AF647 conjugated goat anti-mouse IgG was used as the secondary antibody (red). Cells were counterstained with AF488 conjugated Phalloidin (green).
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![Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)](https://www.abcam.com/ps/products/191/ab191181/Images/ab191181-280636-Human-Nuclear-C.jpg)
Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)Anti-Human Nuclear Antigen antibody [235-1] ab191181 Frozen Human Tonsil fixed in cold acetone, Primary Ab used at 0.5ug/ml for 30 minutes at room temp. Visualised using a HRP-Polymer detection system.
Protocols
References
This product has been referenced in:
- Li W et al. Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model. Stem Cell Res Ther 9:9 (2018). Read more (PubMed: 29335016) »
- Ogawa K et al. Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors. Sci Rep 8:3615 (2018). Read more (PubMed: 29483626) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"