Anti-Human Nuclear Antigen antibody [235-1] (ab191181)

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Immunocytochemistry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
  • Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
  • Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
  • Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
  • Immunocytochemistry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
  • Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
  • Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
  • Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
  • Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)

Key features and details

  • Mouse monoclonal [235-1] to Human Nuclear Antigen
  • Suitable for: ICC, Flow Cyt, IHC-Fr
  • Reacts with: Human, Non human primates
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

PE

Overview

  • Product name

    Anti-Human Nuclear Antigen antibody [235-1]
    See all Human Nuclear Antigen primary antibodies

  • Description

    Mouse monoclonal [235-1] to Human Nuclear Antigen

  • Host species

    Mouse

  • Specificity

    ab191181 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in frozen tissue sections. It can be used with paraformaldehyde-fixed frozen tissue or cell preparations.

  • Tested applications

    Suitable for: ICC, Flow Cyt, IHC-Frmore details

  • Species reactivity

    Reacts with: Human, Non human primates
    Does not react with: Mouse, Rat, Chicken

  • Immunogen

    Tissue, cells or virus corresponding to Human Human Nuclear Antigen. Nuclei of Human myeloid leukemia biopsy cells

  • Positive control

    • IHC-Fr: Human tonsil tissue; ICC: MCF and K562 cells; Flow Cyt: Raji, MCF7, K562, HeLa, and Jurkat cells.

  • General notes

    ab190710 is recommended for IHC-P with paraffin embedded sections.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Monoclonal

  • Clone number

    235-1

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab191181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use a concentration of 1 - 2 µg/ml.
Flow Cyt
Use 1-2µg for 106 cells.
IHC-Fr
Use a concentration of 0.5 - 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Notes
ICC
Use a concentration of 1 - 2 µg/ml.
Flow Cyt
Use 1-2µg for 106 cells.
IHC-Fr
Use a concentration of 0.5 - 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Alternative names

    • HNA antibody
    • HuNu antibody

Images

  • Immunocytochemistry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Immunocytochemistry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    PFA-fixed MCF-7 (human breast adenocarcinoma cell line) cells stained for Human Nuclear Antigen using ab191181 at 2 ug/ml in ICC.
  • Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometric analysis of paraformaldehyde-fixed Raji (human Burkitt's lymphoma cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
  • Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)Rockne et al PLoS One. 2018 Aug 2;13(8):e0199967. doi: 10.1371/journal.pone.0199967. eCollection 2018. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Characterization of LM-NSC008 cells.

    Visualization of LM-NSC008 cells in naïve non-tumor bearing mouse brain 6 and 9 months post administration. (A) Immunohistochemistry (IHC) staining of LM-NSC008 cells (stably expressing eGFP/Ffluc) with anti-HNA antibodies (NSC injection site, white arrows indicate the LM-NSC008 cells, scale bar 100 μm). (1a-1d) Insets show LM-NSC008 cells co-expressing HNA and eGFP proteins (inset scale bars [1a-1f] 2 μm).

     

  • Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)

    Cold acetone-fixed frozen human tonsil tissue stained for Human Nuclear Antigen with ab191181 at 0.5 µg/ml (30 mins, RT) in immunohistochemical analysis. Visualised using a HRP-Polymer detection system.

  • Immunocytochemistry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Immunocytochemistry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow ) cells stained for Human Nuclear Antigen using ab191181 at 2 ug/ml in ICC.
  • Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometric analysis of paraformaldehyde-fixed MCF-7 (human breast adenocarcinoma cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
  • Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometric analysis of paraformaldehyde-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow ) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
  • Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometric analysis of paraformaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
  • Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometric analysis of paraformaldehyde-fixed HeLa (human lymphoblastic leukemia cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

References (31)

Publishing research using ab191181? Please let us know so that we can cite the reference in this datasheet.

ab191181 has been referenced in 31 publications.

  • Choi H  et al. In Vivo Study for Clinical Application of Dental Stem Cell Therapy Incorporated with Dental Titanium Implants. Materials (Basel) 14:N/A (2021). PubMed: 33466799
  • Zhang H  et al. Transplantation of GMP-grade human iPSC-derived retinal pigment epithelial cells in rodent model: the first pre-clinical study for safety and efficacy in China. Ann Transl Med 9:245 (2021). PubMed: 33708872
  • Gong Y  et al. Transplantation of human induced pluripotent stem cell-derived neural crest cells for corneal endothelial regeneration. Stem Cell Res Ther 12:214 (2021). PubMed: 33781330
  • Seiffert N  et al. In vitro recellularization of decellularized bovine carotid arteries using human endothelial colony forming cells. J Biol Eng 15:15 (2021). PubMed: 33882982
  • Thej C  et al. Human bone marrow-derived, pooled, allogeneic mesenchymal stromal cells manufactured from multiple donors at different times show comparable biological functions in vitro, and in vivo to repair limb ischemia. Stem Cell Res Ther 12:279 (2021). PubMed: 33971964
View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Human Nuclear Antigen antibody [235-1]

 Inconclusive
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Xenograft Model, Human Engraftment in murine Pancr)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat Buffer (Vector Laboratories: H-3300)
Permeabilization
No
Specification
Xenograft Model, Human Engraftment in murine Pancr
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jul 26 2019

Western blot abreview for Anti-Human Nuclear Antigen antibody [235-1]

 Inconclusive
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Retinal pigmented epithelium and aortic endotheliu)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
50 µg
Specification
Retinal pigmented epithelium and aortic endotheliu
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jun 18 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Human Nuclear Antigen antibody [235-1]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell
Permeabilization
Yes - 0.25% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Miss. Christian Elabd

Verified customer

Submitted Feb 25 2016

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