Anti-Human Nuclear Antigen antibody [235-1] (ab191181)

Overview

  • Product name
    Anti-Human Nuclear Antigen antibody [235-1]
    See all Human Nuclear Antigen primary antibodies
  • Description
    Mouse Monoclonal [235-1] to Human Nuclear Antigen
  • Host species
    Mouse
  • Specificity
    ab191181 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in frozen tissue sections. It can be used with paraformaldehyde-fixed frozen tissue or cell preparations.
  • Tested applications
    Suitable for: Flow Cyt, ICC, IP, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Human, Non human primates
    Does not react with: Mouse, Rat, Chicken
  • Immunogen

    Tissue, cells or virus corresponding to Human Human Nuclear Antigen . (Immunogen: Nuclei of Human myeloid leukemia biopsy cells).

  • Positive control
    • Human tonsil.
  • General notes

    ab190710 is recommned for IHC-P with paraffin embedded sections.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    235-1
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5-1µg for 106 cells.
ICC Use a concentration of 0.25 - 0.5 µg/ml.

Use Acetone fixed cells.

IP Use at 1-2 µg/mg of lysate.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Alternative names
    • HNA antibody
    • HuNu antibody

Images

  • Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody was used at 1ug/ml for 60 minutes at room temp. AF647 conjugated goat anti-mouse IgG was used as the secondary antibody (red). Cells were counterstained with AF488 conjugated Phalloidin (green).

  • Anti-Human Nuclear Antigen antibody [235-1] ab191181 Frozen Human Tonsil fixed in cold acetone, Primary Ab used at 0.5ug/ml for 30 minutes at room temp. Visualised using a HRP-Polymer detection system.

References

This product has been referenced in:
  • Li W  et al. Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model. Stem Cell Res Ther 9:9 (2018). Read more (PubMed: 29335016) »
  • Ogawa K  et al. Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors. Sci Rep 8:3615 (2018). Read more (PubMed: 29483626) »

See all 8 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Retinal pigmented epithelium and aortic endotheliu)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
50 µg
Specification
Retinal pigmented epithelium and aortic endotheliu
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 18 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell
Permeabilization
Yes - 0.25% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Christian Elabd

Verified customer

Submitted Feb 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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