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    human-nuclear-antigen-antibody-235-1-ab191181.pdf

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Tags & Cell Markers Subcellular Markers Nucleus Chromatin
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Anti-Human Nuclear Antigen antibody [235-1] (ab191181)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (23)

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Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen  antibody [235-1] (ab191181)
  • Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen  antibody [235-1] (ab191181)

Key features and details

  • Mouse monoclonal [235-1] to Human Nuclear Antigen
  • Suitable for: IHC-Fr
  • Reacts with: Human, Non human primates
  • Isotype: IgG1

You may also be interested in

Primary
Product image
PE Anti-Human Nuclear Antigen antibody [235-1] (ab215755)

View more associated products

Overview

  • Product name

    Anti-Human Nuclear Antigen antibody [235-1]
    See all Human Nuclear Antigen primary antibodies
  • Description

    Mouse monoclonal [235-1] to Human Nuclear Antigen
  • Host species

    Mouse
  • Specificity

    ab191181 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in frozen tissue sections. It can be used with paraformaldehyde-fixed frozen tissue or cell preparations.
  • Tested applications

    Suitable for: IHC-Frmore details
  • Species reactivity

    Reacts with: Human, Non human primates
    Does not react with: Mouse, Rat, Chicken
  • Immunogen

    Tissue, cells or virus corresponding to Human Nuclear Antigen. (Immunogen: Nuclei of Human myeloid leukemia biopsy cells).

  • Positive control

    • IHC-Fr: Human tonsil tissue.
  • General notes

    ab190710 is recommended for IHC-P with paraffin embedded sections.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    235-1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Chromatin

Associated products

  • Alternative Versions

    • PE Anti-Human Nuclear Antigen antibody [235-1] (ab215755)
    • Anti-Human Nuclear Antigen antibody [235-1] - BSA and Azide free (ab216943)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Related Products

    • Anti-Nucleolin antibody [364-5] (ab136649)

Applications

Our Abpromise guarantee covers the use of ab191181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Alternative names

    • HNA antibody
    • HuNu antibody

Images

  • Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen  antibody [235-1] (ab191181)
    Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)Rockne et al PLoS One. 2018 Aug 2;13(8):e0199967. doi: 10.1371/journal.pone.0199967. eCollection 2018. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Characterization of LM-NSC008 cells.

    Visualization of LM-NSC008 cells in naïve non-tumor bearing mouse brain 6 and 9 months post administration. (A) Immunohistochemistry (IHC) staining of LM-NSC008 cells (stably expressing eGFP/Ffluc) with anti-HNA antibodies (NSC injection site, white arrows indicate the LM-NSC008 cells, scale bar 100 μm). (1a-1d) Insets show LM-NSC008 cells co-expressing HNA and eGFP proteins (inset scale bars [1a-1f] 2 μm).

     

  • Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen  antibody [235-1] (ab191181)
    Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)

    Cold acetone-fixed frozen human tonsil tissue stained for Human Nuclear Antigen with ab191181 at 0.5 µg/ml (30 mins, RT) in immunohistochemical analysis. Visualised using a HRP-Polymer detection system.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (23)

    Publishing research using ab191181? Please let us know so that we can cite the reference in this datasheet.

    ab191181 has been referenced in 23 publications.

    • Deng R  et al. Haemin pre-treatment augments the cardiac protection of mesenchymal stem cells by inhibiting mitochondrial fission and improving survival. J Cell Mol Med 24:431-440 (2020). PubMed: 31660694
    • Shi Y  et al. Vascularized human cortical organoids (vOrganoids) model cortical development in vivo. PLoS Biol 18:e3000705 (2020). PubMed: 32401820
    • Horowitz LF  et al. Multiplexed drug testing of tumor slices using a microfluidic platform. NPJ Precis Oncol 4:12 (2020). PubMed: 32435696
    • Zhang Y  et al. Macrophage migration inhibitory factor rejuvenates aged human mesenchymal stem cells and improves myocardial repair. Aging (Albany NY) 11:12641-12660 (2019). PubMed: 31881006
    • Xiao M  et al. Synergistic effects of stromal cell-derived factor-1a and bone morphogenetic protein-2 treatment on odontogenic differentiation of human stem cells from apical papilla cultured in the VitroGel 3D system. Cell Tissue Res 378:207-220 (2019). PubMed: 31152245
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Human Nuclear Antigen antibody [235-1]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Xenograft Model, Human Engraftment in murine Pancr)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrat Buffer (Vector Laboratories: H-3300)
    Permeabilization
    No
    Specification
    Xenograft Model, Human Engraftment in murine Pancr
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 26 2019

    Western blot abreview for Anti-Human Nuclear Antigen antibody [235-1]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Retinal pigmented epithelium and aortic endotheliu)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    50 µg
    Specification
    Retinal pigmented epithelium and aortic endotheliu
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 18 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Human Nuclear Antigen antibody [235-1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell
    Permeabilization
    Yes - 0.25% Triton X-100
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Miss. Christian Elabd

    Verified customer

    Submitted Feb 25 2016

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