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Tissue, cells or virus corresponding to Human Human Nuclear Antigen . (Immunogen: Nuclei of Human myeloid leukemia biopsy cells).
ab190710 is recommned for IHC-P with paraffin embedded sections.
Our Abpromise guarantee covers the use of ab191181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.5-1µg for 106 cells.|
|ICC||Use a concentration of 0.25 - 0.5 µg/ml.
Use Acetone fixed cells.
|IP||Use at 1-2 µg/mg of lysate.|
|ICC/IF||Use a concentration of 0.5 - 1 µg/ml.|
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody was used at 1ug/ml for 60 minutes at room temp. AF647 conjugated goat anti-mouse IgG was used as the secondary antibody (red). Cells were counterstained with AF488 conjugated Phalloidin (green).
Anti-Human Nuclear Antigen antibody [235-1] ab191181 Frozen Human Tonsil fixed in cold acetone, Primary Ab used at 0.5ug/ml for 30 minutes at room temp. Visualised using a HRP-Polymer detection system.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"