Anti-Human Nuclear Antigen antibody [NM106] - Nuclear Marker (ab215396)

Overview

  • Product name
    Anti-Human Nuclear Antigen antibody [NM106] - Nuclear Marker
    See all Human Nuclear Antigen primary antibodies
  • Description
    Mouse monoclonal [NM106] to Human Nuclear Antigen - Nuclear Marker
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: MouseDoes not react with: Pig
  • Immunogen

    Tissue, cells or virus corresponding to Human Nuclear Antigen. (Nuclei of HL60 cells).

  • Positive control
    • Human tonsil tissue; Rat lung and colon tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab215396 was purified from Bioreactor Concentrate by Protein A/G.
  • Clonality
    Monoclonal
  • Clone number
    NM106
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab215396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(Primary incubation for 30 minutes at RT).

Target

  • Alternative names
    • HNA antibody
    • HuNu antibody

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Human Nuclear Antigen with ab215396 at 0.5 μg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Human Nuclear Antigen with ab215396 at 0.5 μg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat colon tissue labeling Human Nuclear Antigen with ab215396 at 0.5 μg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling Human Nuclear Antigen with ab215396 at 0.5 μg/ml.

References

ab215396 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (hESC derived RPE cell)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: EDTA
Permeabilization
No
Specification
hESC derived RPE cell
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 28 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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