Anti-Human Nuclear Antigen antibody [NM106] - Nuclear Marker (ab215396)
Key features and details
- Mouse monoclonal [NM106] to Human Nuclear Antigen - Nuclear Marker
- Suitable for: IHC-P, Flow Cyt, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Human Nuclear Antigen antibody [NM106] - Nuclear Marker
See all Human Nuclear Antigen primary antibodies -
Description
Mouse monoclonal [NM106] to Human Nuclear Antigen - Nuclear Marker -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: MouseDoes not react with: Pig -
Immunogen
Tissue, cells or virus corresponding to Human Nuclear Antigen. (Nuclei of HL60 cells).
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Positive control
- IHC: Human tonsil tissue; Rat lung and colon tissues. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab215396 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
NM106 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT). Perform heat mediated antigen retrieval with 10mM Tris with 1mM EDTA, pH 9, for 45 min at 95°C followed by cooling at RT for 20 minutes before commencing with IHC staining protocol. |
Flow Cyt |
Use 1-2µg for 106 cells.
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ICC/IF |
Use a concentration of 1 - 2 µg/ml.
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Notes |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Primary incubation for 30 minutes at RT). Perform heat mediated antigen retrieval with 10mM Tris with 1mM EDTA, pH 9, for 45 min at 95°C followed by cooling at RT for 20 minutes before commencing with IHC staining protocol. |
Flow Cyt
Use 1-2µg for 106 cells. |
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
Target
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Alternative names
- HNA antibody
- HuNu antibody
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Human Nuclear Antigen with ab215396 at 0.5 μg/ml.
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Immunocytochemistry analysis of HeLa cells labelling Human Nuclear Antigen antibody with ab215396 at 2 µg/mL. Goat Anti-mouse IgG (Alexa Fluor® 488) was used as the secondary antibody (green). Membranes were labeled with Phalloidin (Red).
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Human Nuclear Antigen with ab215396 at 0.5 μg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat colon tissue labeling Human Nuclear Antigen with ab215396 at 0.5 μg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling Human Nuclear Antigen with ab215396 at 0.5 μg/ml.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab215396 has been referenced in 1 publication.
- Xie P et al. Therapeutic effect of transplantation of human bone marrow-derived mesenchymal stem cells on neuron regeneration in a rat model of middle cerebral artery occlusion. Mol Med Rep 20:3065-3074 (2019). PubMed: 31432152