Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Human Nucleoli antibody [NM95] - BSA and Azide free
See all Human Nucleoli primary antibodies -
Description
Mouse monoclonal [NM95] to Human Nucleoli - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Cow -
Immunogen
Tissue, cells or virus corresponding to Human Nucleoli. Nuclei of myeloid leukemia biopsy cells.
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Positive control
- IHC-P: Human skin, tonsil and colon carcinoma tissue. Flow: HEK-293T cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
NM95 -
Isotype
IgG1 -
Light chain type
kappa
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab234031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 0.25 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 30 minutes at RT. |
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| Flow Cyt | Use at an assay dependent concentration. |
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)](https://www.abcam.com/ps/products/234/ab234031/Images/ab234031-308086-0311.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)Formalin-fixed, paraffin-embedded human skin tissue stained for human nucleoli using ab234031 at 0.25 μg/ml in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)](https://www.abcam.com/ps/products/234/ab234031/Images/ab234031-308087-0312.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)Formalin-fixed, paraffin-embedded human tonsil tissue stained for human nucleoli using ab234031 at 0.25 μg/ml in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)](https://www.abcam.com/ps/products/234/ab234031/Images/ab234031-308088-0313.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for human nucleoli using Alexa Fluor488-conjugated ab234031 at 0.25 μg/ml in immunohistochemical analysis.
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![Flow Cytometry - Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)](https://www.abcam.com/ps/products/234/ab234031/Images/ab234031-308089-0314.jpg)
Flow Cytometry - Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling human nucleoli using Alexa Fluor488-labeled ab234031 (green), compared with cells alone (black) and an isotype control (grey).
Datasheets and documents
References
ab234031 has not yet been referenced specifically in any publications.
