Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)](https://www.abcam.com/ps/products/234/ab234031/Images/ab234031-308086-0311.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)")
Key features and details
- Mouse monoclonal [NM95] to Human Nucleoli - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Human Nucleoli antibody [NM95] - BSA and Azide free
See all Human Nucleoli primary antibodies -
Description
Mouse monoclonal [NM95] to Human Nucleoli - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Cow -
Immunogen
Tissue, cells or virus corresponding to Human Nucleoli. Nuclei of myeloid leukemia biopsy cells.
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Positive control
- IHC-P: Human skin, tonsil and colon carcinoma tissue. Flow: HEK-293T cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
NM95 -
Isotype
IgG1 -
Light chain type
kappa
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab234031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 0.25 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 30 minutes at RT. |
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| Flow Cyt | Use at an assay dependent concentration. |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)
Formalin-fixed, paraffin-embedded human skin tissue stained for human nucleoli using ab234031 at 0.25 μg/ml in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)](https://www.abcam.com/ps/products/234/ab234031/Images/ab234031-308087-0312.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)Formalin-fixed, paraffin-embedded human tonsil tissue stained for human nucleoli using ab234031 at 0.25 μg/ml in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)](https://www.abcam.com/ps/products/234/ab234031/Images/ab234031-308088-0313.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for human nucleoli using Alexa Fluor488-conjugated ab234031 at 0.25 μg/ml in immunohistochemical analysis.
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![Flow Cytometry - Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)](https://www.abcam.com/ps/products/234/ab234031/Images/ab234031-308089-0314.jpg)
Flow Cytometry - Anti-Human Nucleoli antibody [NM95] - BSA and Azide free (ab234031)Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling human nucleoli using Alexa Fluor488-labeled ab234031 (green), compared with cells alone (black) and an isotype control (grey).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab234031 has not yet been referenced specifically in any publications.
