Anti-Human Nucleoli antibody [NM95] - Nucleolar Marker (ab190710)

Overview

  • Product name

    Anti-Human Nucleoli antibody [NM95] - Nucleolar Marker
    See all Human Nucleoli primary antibodies
  • Description

    Mouse monoclonal [NM95] to Human Nucleoli - Nucleolar Marker
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, IHC-Fr, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Nucleoli. Nuclei of myeloid leukemia biopsy cells

  • Positive control

    • IHC-P: Human skin, tonsil and colon carcinoma tissues.
  • General notes

    ab190710 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.05% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    NM95
  • Isotype

    IgG1
  • Light chain type

    kappa

Applications

Our Abpromise guarantee covers the use of ab190710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.

Acetone-fixed cells

ICC/IF Use a concentration of 0.5 - 1 µg/ml.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Images

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Human Nucleoli using ab190710 at 1 µg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human Tonsil stained with ab190710 at 1 μg/ml

  • Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Human Nucleoli using ab190710 at 1 µg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with ab190710 at 1 μg/ml

  • Immunohistochemical analysis of paraffin embedded Human tonsil labelling nuclei with ab190710 at 1.0 μg/ml.

  • Flow Cytometry of Human Nucleolar Marker on 293T Cells.

    Black: Cells alone; Grey: Isotype Control;

    Green: AF488-labeled Human Nucleoli antibody (ab190710).

References

This product has been referenced in:

  • Justus CR  et al. Contextual tumor suppressor function of T cell death-associated gene 8 (TDAG8) in hematological malignancies. J Transl Med 15:204 (2017). IHC . Read more (PubMed: 29017562) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Teratoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Teratoma
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Jul 27 2018

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