Recombinant Anti-Human Nucleoli antibody [NM95] - Nucleolar Marker (ab190710)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [NM95] to Human Nucleoli - Nucleolar Marker
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Human Nucleoli antibody [NM95] - Nucleolar Marker
See all Human Nucleoli primary antibodies -
Description
Mouse monoclonal [NM95] to Human Nucleoli - Nucleolar Marker -
Host species
Mouse -
Specificity
- Recommend: ICC[Hu], Flow[Hu], WB[Hu], IHC-P[Hu]
- Do not recommend: WB[Ms, Rt], IHC-P[Ms,Rt] -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Nucleoli. Nuclei of myeloid leukemia biopsy cells
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Positive control
- WB: HeLa and K562 whole cell lysate. IHC-P: Human kidney tissue; Human colon cancer tissue. ICC/IF: K562 and HeLa cells. Flow Cyt (Intra): K562 cells.
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General notes
This product has switched from a hybridoma to recombinant production method on 22nd July 2021.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
NM95 -
Isotype
IgG1
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/100.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 100 kDa.
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IHC-P | (1) |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
1/1000.
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Notes |
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ICC/IF
1/50 - 1/100. |
WB
1/1000 - 1/10000. Predicted molecular weight: 100 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
1/1000. |
Images
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All lanes : Anti-Human Nucleoli antibody [NM95] - Nucleolar Marker (ab190710) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 100 kDaExposure Times
lane 1: 3 seconds
lane 2: 5 seconds5% NFDM/TBST
Lysates were made freshly and used in WB immediately to minimize protein degradation.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling Human Nucleoli with ab190710 at 1/1000 dilution, followed by secondary antibody the ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining in human kidney is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
The section was incubated with ab190710 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
Immunofluorescent analysis of 100% methanol, 0.1% TritonX-100 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Human Nucleoli with ab190710 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleolar staining in HeLa cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-beta Tubulin rabbit monoclonal antibody (ab179513) at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab190710 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) secondary at 1/500 dilution.
-ve control 2: Anti-beta Tubulin rabbit monoclonal antibody (ab179513) at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) secondary at 1/1000 dilution. -
Immunofluorescent analysis of 100% methanol, 0.1% TritonX-100 permeabilized K562 (Human chronic myelogenous leukemia lymphoblast cell line ) cells labelling Human Nucleoli with ab190710 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleolar staining in K562 cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution (red).
Tubulin is detected with Anti-beta Tubulin rabbit monoclonal antibody (ab179513) at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab190710 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (abab150080) secondary at 1/1000 dilution.
-ve control 2: Anti-beta Tubulin rabbit monoclonal antibody (ab179513) at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) secondary at 1/1000 dilution. -
Immunohistochemical analysis of paraffin-embedded sections human colon cancer tissue labelling Human Nucleoli with ab190710 at 1/2000 dilution, followed by secondary ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining in human colon cancer tissue is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
The section was incubated with ab190710 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
Flow cytometry overlay histogram of 4% paraformaldehyde-fixed, 90% methanol permeabilized K562 (Human chronic myelogenous leukemia lymphoblast) cells labelling Human Nucleoli with ab190710 at 1/1000 dilution (red) compared with a Mouse monoclonal IgG (black) and an unlabelled control (Cell without incubation with primary antibody and secondary antibody) (blue). Goat anti mouse IgG (Alexa Fluor® 488) (ab150113), at 1/2000 dilution was used as the secondary antibody.
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab190710 has been referenced in 15 publications.
- Hong Y et al. Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplastica. Nat Commun 13:1309 (2022). PubMed: 35288560
- Miyata R et al. Lung fibrogenic microenvironment in mouse reconstitutes human alveolar structure and lung tumor. iScience 25:104912 (2022). PubMed: 36060050
- Wilson MN et al. Multimodal monitoring of human cortical organoids implanted in mice reveal functional connection with visual cortex. Nat Commun 13:7945 (2022). PubMed: 36572698
- Piao J et al. Preclinical Efficacy and Safety of a Human Embryonic Stem Cell-Derived Midbrain Dopamine Progenitor Product, MSK-DA01. Cell Stem Cell 28:217-229.e7 (2021). PubMed: 33545080
- Thomas HM et al. Plasma-polymerized pericyte patches improve healing of murine wounds through increased angiogenesis and reduced inflammation. Regen Biomater 8:rbab024 (2021). PubMed: 34221447