Overview

  • Product name
    Human Nucleolin peptide

Description

  • Nature
    Synthetic
  • Amino Acid Sequence
    • Species
      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab25315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Nucleolin antibody (ab22758)

  • Form
    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • C23
    • FLJ45706
    • MS1116
    • NCL
    • Nucl
    • NUCL_HUMAN
    • Nucleolin
    • Protein C23
    see all
  • Function
    Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
  • Sequence similarities
    Contains 4 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • Cellular localization
    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt

References

This product has been referenced in:
  • Li Z  et al. Aptamer-Capped Multifunctional Mesoporous Strontium Hydroxyapatite Nanovehicle for Cancer-Cell-Responsive Drug Delivery and Imaging. Biomacromolecules : (2012). Read more (PubMed: 23140615) »
  • Fabricius EM  et al. Immunohistochemical determination of the appropriate anti-hTERT antibodies for in situ detection of telomerase activity in frozen sections of head and neck squamous cell carcinomas and tumor margin tissues. Int J Oncol 34:1257-79 (2009). Blocking . Read more (PubMed: 19360339) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your reply.

Although the antibody from Santa Cruz, MS-3 was raised against the full length protein, because it is a monoclonal antibody it will only recognize a single epitope on the protein. There is a very good chance that the peptide ab25315 will not be recognized by that antibody. If the epitope for the antibody has been mapped, you can try to find a shorter peptide that includes the epitope. Otherwise, your best bet will be to find a purified full length protein, but unfortunately we do not currently have one in our catalog. You could try searching biocompare.com

I hope this helps, please let me know if you need any additional information or assistance.

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Answer

Thank you for your reply.

I am not sure what catalog number you mean by "MS3" but if the antibody is a polyclonal raised against the full length protein it is likely to show weak reactivity with the peptide ab25315 because only a fraction of the antibodies will detect epitopes in that region of the protein. If the antibody is a monoclonal, it may or may not react with the peptide, depending on where the epitope is mapped to. Ab50279 should show some reactivity with this peptide, but these products have not been tested for use together.

If you could please let me know what you are looking for, I will be happy to help you further. Are you searching for an antibody that would be blocked by this peptide? Or a protein to use as a positive control? Does the antibody need to react with a specific species or be validated in a particular application?

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Answer

Thank you for contacting us.

The peptide ab25315 is a 14 amino acid peptide from within residues 1-100 of human Nucleolin. The exact sequence is considered to be proprietary because this peptide was used as an immunogen for ab22758. The 706 amino acid length refers to the whole protein sequence, and is provided for reference.
I hope this helps, please let me know if you need any additional information or assistance.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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