Human Nucleoside Diphosphate Kinase D ELISA Kit (ab283968)
Key features and details
- Sensitivity: 0.34 ng/ml
- Range: 0.75 ng/ml - 24 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Nucleoside Diphosphate Kinase D ELISA Kit
See all NME4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 60 5.6% Inter-assay Sample n Mean SD CV% Overall 60 10.2% -
Sample type
Cell culture supernatant, Serum, Plasma, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
0.34 ng/ml -
Range
0.75 ng/ml - 24 ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human Nucleoside Diphosphate Kinase D ELISA Kit (ab283968) is designed for detection of Nucleoside Diphosphate Kinase D (NDPKD) in human plasma, serum, cell culture, and cell lysate samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures human NDPKD in approximately 5 hours. A polyclonal antibody specific for human NDPKD has been pre-coated onto a 96-well microplate with removable strips. NDPKD in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human NDPKD, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 20ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml Chromogen Substrate 1 vial Human Nucleoside Diphosphate Kinase D Microplate 1 unit Nucleoside Diphosphate Kinase D Standard 1 vial Sealing Tapes 3 units Stop Solution 1 vial 50X Biotinylated Human Nucleoside Diphosphate Kinase D Antibody 1 vial -
Research areas
-
Function
Major role in the synthesis of nucleoside triphosphates other than ATP. -
Tissue specificity
Widely distributed. Found at very high levels in prostate, heart, liver, small intestine, and skeletal muscle tissues, and in low amounts in the brain and in blood leukocytes. -
Sequence similarities
Belongs to the NDK family. -
Cellular localization
Mitochondrion intermembrane space. - Information by UniProt
-
Alternative names
- metastatic inhibition factor NM23H4
- mitochondrial
- NDK
see all -
Database links
- Entrez Gene: 4833 Human
- Omim: 601818 Human
- SwissProt: O00746 Human
- Unigene: 9235 Human
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab283968 has not yet been referenced specifically in any publications.