Human Obesity Antibody Array - Membrane (62 targets) (ab169819)

Q&A (1)References (2)
Muliplex Protein Detection - Human Obesity Antibody Array - Membrane (62 targets) (ab169819)
  • Multiplex Protein Detection - Human Obesity Antibody Array - Membrane (62 targets) (ab169819)

Overview

  • Product name

    Human Obesity Antibody Array - Membrane (62 targets)
    See all Obesity Antibody Array antibody arrays

  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media

  • Assay type

    Semi-quantitative

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam's Human Obesity Antibody Array - Membrane (62 targets) detects 62 Human Adipokines in 4 or 8 samples. Suitable for all liquid sample types.


    Targets:4-1BB, ACE-2, Adiponectin, Adipsin, AgRP, Angiopoietin 1, Angiopoietin 2, ANGPTL4, CRP, ENA-78, Fas, FGF-6, Growth Hormone, HCC-4, IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGF-1, IGF-1 sR, IL-1 R4, IL-1 sRI, IL-10, IL-11, IL-12, IL-1 alpha, IL-1 beta, IL-6, IL-6 sR, IL-8, Insulin, IP-10, Leptin R, Leptin, LIF, Lymphotactin, MCP-1, MCP-3, M-CSF, MIF, MIP-1 beta, MSP-alpha, Osteoprotegerin, OSM , PAI-I, PARC, PDGF-AA, PDGF-AB, PDGF-BB, RANTES, Resistin, Serum Amyloid A , SDF-1 , sTNFRII, sTNFRI, TECK, TGF beta 1, TIMP-1, TIMP-2, TNF alpha, VEGF-A, XEDAR


    Learn more about membrane antibody arrays

  • Notes

    If you are interested in this cytokine array, arrays ab133998 and ab134000 may also be of interest. A list of human antibody membrane arrays is available here.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Tested applications

    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes
    1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl
    1X Blocking Buffer 1 x 25ml 2 x 25ml
    2000X Biotin-Conjugated Anti Adipokines 2 vials 4 vials
    20X Wash Buffer I 1 x 10ml 1 x 20ml
    20X Wash Buffer II 1 x 10ml 1 x 20ml
    2X Cell Lysis Buffer 1 x 10ml 1 x 16ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit
    Detection Buffer C 1 x 1.5ml 1 x 2.5ml
    Detection Buffer D 1 x 1.5ml 1 x 2.5ml
    Obesity Antibody Array Membranes 4 units 8 units

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab169819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection
Use at an assay dependent concentration.
Notes
Multiplex Protein Detection
Use at an assay dependent concentration.

Images

  • Muliplex Protein Detection - Human Obesity Antibody Array - Membrane (62 targets) (ab169819)
    Muliplex Protein Detection - Human Obesity Antibody Array - Membrane (62 targets) (ab169819)This image is courtesy of an anonymous Abreview

    5 mg of lyophilized microdissected skeletal muscle (vastus laterals) was homogenized in 180 uL 1X cell lysis buffer (included in kit). After a 10,000 x g 5 min spin at 4°C removed the supernatant was removed and the total protein determined. 250 µg of total protein was added to each membrane with overnight incubations for each incubation step. The attached image is the first blot setting up the method and was exposed using a FluorChem M for 27 seconds. As can be seen Acrp30 will require less protein/less exposure but otherwise all but seven cytokines were visualized following this protocol.

    Abreview rating 5/5 stars. Review from Abcam user community. Verified customer at Baltimore VA Medical Center. Submitted Oct 9 2014.

  • Multiplex Protein Detection - Human Obesity Antibody Array - Membrane (62 targets) (ab169819)
    Multiplex Protein Detection - Human Obesity Antibody Array - Membrane (62 targets) (ab169819)
    Typical images obtained with Abcam Human Cytokine Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

References (2)

Publishing research using ab169819? Please let us know so that we can cite the reference in this datasheet.

ab169819 has been referenced in 2 publications.

  • Serra MC  et al. Resistance training reduces inflammation and fatigue and improves physical function in older breast cancer survivors. Menopause 25:211-216 (2018). PubMed: 28832427
  • Mustapic M  et al. Plasma Extracellular Vesicles Enriched for Neuronal Origin: A Potential Window into Brain Pathologic Processes. Front Neurosci 11:278 (2017). PubMed: 28588440

Customer reviews and Q&As

Q&A
Submit a question

Question

I know this array is for use with human protein. But I'm looking for a product like this to use with mouse samples so I'd like to know which, if any, of the antibodies on this array have been successfully tested to react with mouse proteins.

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Answer

There is likely cross reactivity with at least a portion of the antibodies on the array to mouse protein, but the antibodies have not been tested in this array format for reactivity to mouse. Without running a positive control for each antibody on the array, it may be difficult to determine to what degree there is cross reactivity.

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