Human Obesity Antibody Array - Membrane (62 targets) (ab169819)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Human Obesity Antibody Array - Membrane (62 targets)
See all Obesity Antibody Array antibody arrays -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Semi-quantitative -
Species reactivity
Reacts with: Human -
Product overview
Abcam's Human Obesity Antibody Array - Membrane (62 targets) detects 62 Human Adipokines in 4 or 8 samples. Suitable for all liquid sample types.
Targets:4-1BB, ACE-2, Adiponectin, Adipsin, AgRP, Angiopoietin 1, Angiopoietin 2, ANGPTL4, CRP, ENA-78, Fas, FGF-6, Growth Hormone, HCC-4, IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGF-1, IGF-1 sR, IL-1 R4, IL-1 sRI, IL-10, IL-11, IL-12, IL-1 alpha, IL-1 beta, IL-6, IL-6 sR, IL-8, Insulin, IP-10, Leptin R, Leptin, LIF, Lymphotactin, MCP-1, MCP-3, M-CSF, MIF, MIP-1 beta, MSP-alpha, Osteoprotegerin, OSM , PAI-I, PARC, PDGF-AA, PDGF-AB, PDGF-BB, RANTES, Resistin, Serum Amyloid A , SDF-1 , sTNFRII, sTNFRI, TECK, TGF beta 1, TIMP-1, TIMP-2, TNF alpha, VEGF-A, XEDAR
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Notes
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Tested applications
Suitable for: Multiplex Protein Detectionmore details
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 2000X Biotin-Conjugated Anti Adipokines 2 vials 4 vials 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml Obesity Antibody Array Membranes 4 units 8 units -
Research areas
Applications
Our Abpromise guarantee covers the use of ab169819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Multiplex Protein Detection | Use at an assay dependent concentration. |
Images
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Muliplex Protein Detection - Human Obesity Antibody Array - Membrane (62 targets) (ab169819)This image is courtesy of an anonymous Abreview5 mg of lyophilized microdissected skeletal muscle (vastus laterals) was homogenized in 180 uL 1X cell lysis buffer (included in kit). After a 10,000 x g 5 min spin at 4°C removed the supernatant was removed and the total protein determined. 250 µg of total protein was added to each membrane with overnight incubations for each incubation step. The attached image is the first blot setting up the method and was exposed using a FluorChem M for 27 seconds. As can be seen Acrp30 will require less protein/less exposure but otherwise all but seven cytokines were visualized following this protocol.
Abreview rating 5/5 stars. Review from Abcam user community. Verified customer at Baltimore VA Medical Center. Submitted Oct 9 2014.
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Multiplex Protein Detection - Human Obesity Antibody Array - Membrane (62 targets) (ab169819)Typical images obtained with Abcam Human Cytokine Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.
Protocols
References
This product has been referenced in:
- Serra MC et al. Resistance training reduces inflammation and fatigue and improves physical function in older breast cancer survivors. Menopause 25:211-216 (2018). Read more (PubMed: 28832427) »
- Mustapic M et al. Plasma Extracellular Vesicles Enriched for Neuronal Origin: A Potential Window into Brain Pathologic Processes. Front Neurosci 11:278 (2017). Read more (PubMed: 28588440) »
