Overview

  • Product name
    Human Obesity Antibody Array - Membrane (62 targets)
    See all Obesity Antibody Array antibody arrays
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Semi-quantitative
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam's Human Obesity Antibody Array - Membrane (62 targets) detects 62 Human Adipokines in 4 or 8 samples. Suitable for all liquid sample types.


    Targets:4-1BB, ACE-2, Adiponectin, Adipsin, AgRP, Angiopoietin 1, Angiopoietin 2, ANGPTL4, CRP, ENA-78, Fas, FGF-6, Growth Hormone, HCC-4, IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGF-1, IGF-1 sR, IL-1 R4, IL-1 sRI, IL-10, IL-11, IL-12, IL-1 alpha, IL-1 beta, IL-6, IL-6 sR, IL-8, Insulin, IP-10, Leptin R, Leptin, LIF, Lymphotactin, MCP-1, MCP-3, M-CSF, MIF, MIP-1 beta, MSP-alpha, Osteoprotegerin, OSM , PAI-I, PARC, PDGF-AA, PDGF-AB, PDGF-BB, RANTES, Resistin, Serum Amyloid A , SDF-1 , sTNFRII, sTNFRI, TECK, TGF beta 1, TIMP-1, TIMP-2, TNF alpha, VEGF-A, XEDAR


    Learn more about membrane antibody arrays

  • Notes

    If you are interested in this cytokine array, arrays ab133998 and ab134000 may also be of interest. A list of human antibody membrane arrays is available here.

  • Tested applications
    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes
    1,000X HRP-Streptavidin Buffer 1 x 50µl 1 x 50µl
    1X Blocking Buffer 1 x 25ml 2 x 25ml
    2000X Biotin-Conjugated Anti Adipokines 2 vials 4 vials
    20X Wash Buffer I 1 x 10ml 1 x 20ml
    20X Wash Buffer II 1 x 10ml 1 x 20ml
    2X Cell Lysis Buffer 1 x 10ml 1 x 16ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit
    Detection Buffer C 1 x 1.5ml 1 x 2.5ml
    Detection Buffer D 1 x 1.5ml 1 x 2.5ml
    Obesity Antibody Array Membranes 4 units 8 units
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • 5 mg of lyophilized microdissected skeletal muscle (vastus laterals) was homogenized in 180 uL 1X cell lysis buffer (included in kit). After a 10,000 x g 5 min spin at 4°C removed the supernatant was removed and the total protein determined. 250 µg of total protein was added to each membrane with overnight incubations for each incubation step. The attached image is the first blot setting up the method and was exposed using a FluorChem M for 27 seconds. As can be seen Acrp30 will require less protein/less exposure but otherwise all but seven cytokines were visualized following this protocol.

    Abreview rating 5/5 stars. Review from Abcam user community. Verified customer at Baltimore VA Medical Center. Submitted Oct 9 2014.

  • Typical images obtained with Abcam Human Cytokine Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

Protocols

References

This product has been referenced in:
  • Serra MC  et al. Resistance training reduces inflammation and fatigue and improves physical function in older breast cancer survivors. Menopause 25:211-216 (2018). Read more (PubMed: 28832427) »
  • Mustapic M  et al. Plasma Extracellular Vesicles Enriched for Neuronal Origin: A Potential Window into Brain Pathologic Processes. Front Neurosci 11:278 (2017). Read more (PubMed: 28588440) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

There is likely cross reactivity with at least a portion of the antibodies on the array to mouse protein, but the antibodies have not been tested in this array format for reactivity to mouse. Without running a positive control for each antibody on the array, it may be difficult to determine to what degree there is cross reactivity.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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