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Abcam's Human Obesity Antibody Array - Membrane (62 targets) detects 62 Human Adipokines in 4 or 8 samples. Suitable for all liquid sample types.
Targets:4-1BB, ACE-2, Adiponectin, Adipsin, AgRP, Angiopoietin 1, Angiopoietin 2, ANGPTL4, CRP, ENA-78, Fas, FGF-6, Growth Hormone, HCC-4, IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGF-1, IGF-1 sR, IL-1 R4, IL-1 sRI, IL-10, IL-11, IL-12, IL-1 alpha, IL-1 beta, IL-6, IL-6 sR, IL-8, Insulin, IP-10, Leptin R, Leptin, LIF, Lymphotactin, MCP-1, MCP-3, M-CSF, MIF, MIP-1 beta, MSP-alpha, Osteoprotegerin, OSM , PAI-I, PARC, PDGF-AA, PDGF-AB, PDGF-BB, RANTES, Resistin, Serum Amyloid A , SDF-1 , sTNFRII, sTNFRI, TECK, TGF beta 1, TIMP-1, TIMP-2, TNF alpha, VEGF-A, XEDAR
|Components||1 x 4 Membranes||1 x 8 Membranes|
|1,000X HRP-Streptavidin Buffer||1 x 50µl||1 x 50µl|
|1X Blocking Buffer||1 x 25ml||2 x 25ml|
|2000X Biotin-Conjugated Anti Adipokines||2 vials||4 vials|
|20X Wash Buffer I||1 x 10ml||1 x 20ml|
|20X Wash Buffer II||1 x 10ml||1 x 20ml|
|2X Cell Lysis Buffer||1 x 10ml||1 x 16ml|
|8-Well Incubation Tray (with Lid)||1 unit||1 unit|
|Detection Buffer C||1 x 1.5ml||1 x 2.5ml|
|Detection Buffer D||1 x 1.5ml||1 x 2.5ml|
|Obesity Antibody Array Membranes||4 units||8 units|
Our Abpromise guarantee covers the use of ab169819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Multiplex Protein Detection||Use at an assay dependent concentration.|
5 mg of lyophilized microdissected skeletal muscle (vastus laterals) was homogenized in 180 uL 1X cell lysis buffer (included in kit). After a 10,000 x g 5 min spin at 4°C removed the supernatant was removed and the total protein determined. 250 µg of total protein was added to each membrane with overnight incubations for each incubation step. The attached image is the first blot setting up the method and was exposed using a FluorChem M for 27 seconds. As can be seen Acrp30 will require less protein/less exposure but otherwise all but seven cytokines were visualized following this protocol.
Abreview rating 5/5 stars. Review from Abcam user community. Verified customer at Baltimore VA Medical Center. Submitted Oct 9 2014.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"