Human Oct4 peptide (ab20650)

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Description

Specifications

Our Abpromise guarantee covers the use of ab20650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Oct4 antibody - ChIP Grade (ab19857), Anti-Oct4 antibody (ab27985)

  • Form

    Liquid

  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

    Information available upon request.

General Info

  • Alternative names

    • Octamer binding transcription factor 4
    • MGC22487
    • Oct 3
    • Oct 4
    • Oct-3
    • Oct-4
    • OCT3
    • Oct4
    • Octamer binding protein 3
    • Octamer binding protein 4
    • Octamer binding transcription factor 3
    • Octamer-binding protein 3
    • Octamer-binding protein 4
    • Octamer-binding transcription factor 3
    • OTF 3
    • OTF 4
    • OTF-3
    • OTF3
    • OTF4
    • PO5F1_HUMAN
    • POU class 5 homeobox 1
    • POU domain class 5 transcription factor 1
    • POU domain transcription factor OCT4
    • POU domain, class 5, transcription factor 1
    • POU-type homeodomain-containing DNA-binding protein
    • POU5F1
    see all

  • Function

    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

  • Tissue specificity

    Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

  • Sequence similarities

    Belongs to the POU transcription factor family. Class-5 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.

  • Developmental stage

    Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.

  • Domain

    The POU-specific domain mediates interaction with PKM2.

  • Post-translational
    modifications

    Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.

  • Cellular localization

    Nucleus. Expressed in a diffuse and slightly punctuate pattern.
  • Information by UniProt

Images

  • Western blot - Human Oct4 peptide (ab20650)
    Western blot - Human Oct4 peptide (ab20650)

    Anti-Oct4 antibody ab19857 only detected a band corresponding to the expected size of Oct-4 in human ES cell lysate. In mouse ES and EG cell lysates, ab19857 detected a band of approximately 55 kDa in addition to the expected 39 kDa Oct-4 band.

References

This product has been referenced in:

  • Leopardo NP & Vitullo AD Early embryonic development and spatiotemporal localization of mammalian primordial germ cell-associated proteins in the basal rodent Lagostomus maximus. Sci Rep 7:594 (2017). Read more (PubMed: 28377629) »
  • Salmina K  et al. Up-regulation of the embryonic self-renewal network through reversible polyploidy in irradiated p53-mutant tumour cells. Exp Cell Res 316:2099-112 (2010). Blocking . Read more (PubMed: 20457152) »
See all 2 Publications for this product

Publishing research using ab20650? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

I have an enquiry regarding your products; ab20650 and ab19857.
I would like to confirm with you that ab20650 can be used to elute
Oct4 proteins immunoprecipitated by ab19857.
I would be most grateful if you could allow me yo know
whether it is possible.

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Answer

Thank you for contacting us.

The peptide ab20650 has not been specifically validated for use eluting proteins that were immunoprecipitated using ab19857, but it should be suitable for this purpose when used at a high enough concentration. I would recommend allowing the column to drain completely and elutingthe bound protein with five one-column volume aliquots of a solution containing 100ug / mL peptide in TBS. I hope this helps, please let me know if you have any additional questions.

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