Human on human IHC kit (AP/Permanent Red) (ab214753)

Overview

  • Product name

    Human on human IHC kit (AP/Permanent Red)
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Human on human IHC kit (AP/Permanent Red) (ab214753) is designed for generating staining with the alkaline phosphatase (AP) enzyme of human primary antibodies on human tissues without background staining.


    Antigen detection using a primary antibody of the same species as the test tissue yields high background when an indirect detection method is used. This severely limits the ability to screen human antibodies on human tissues. Abcam’s Human on human IHC kit (AP/Permanent Red) (ab214753) is a ready to use system that results in excellent sensitivity and high specificity using a simple protocol. Furthermore, as the protocol uses a polymer-based, biotin-free system, it does not require blocking of endogenous biotin.


    Note: This kit is recommended for cytoplasmic and membrane bound antigens other patterns of staining have not tested.

  • Tested applications

    Suitable for: IHCmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 6 ml 18 ml 110 ml
    100X Permanent Red Chromogen 1 x 70µl 1 x 180µl 1 x 1.2ml
    5X Permanent Red Activator 1 x 1.4ml 1 x 3.6ml 2 x 12ml
    5X Quenching Buffer 1 x 1.5ml 2 x 2.3ml 2 x 13ml
    Blocking Buffer A 1 x 6ml 1 x 18ml 1 x 110ml
    Blocking Buffer B 1 x 6ml 1 x 18ml 1 x 110ml
    Human AP Polymer 1 x 6ml 1 x 18ml 1 x 110ml
    Human Primer 1 x 6ml 1 x 18ml 1 x 110ml
    Permanent Red Substrate 1 x 7ml 1 x 18ml 1 x 110ml

Associated products

Applications

Our Abpromise guarantee covers the use of ab214753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC Use at an assay dependent concentration.

Protocols

References

ab214753 has not yet been referenced specifically in any publications.

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