Product nameHuman on human IHC kit (AP/Permanent Red)
Species reactivityReacts with: Human
Abcam’s Human on human IHC kit (AP/Permanent Red) (ab214753) is designed for generating staining with the alkaline phosphatase (AP) enzyme of human primary antibodies on human tissues without background staining.
Antigen detection using a primary antibody of the same species as the test tissue yields high background when an indirect detection method is used. This severely limits the ability to screen human antibodies on human tissues. Abcam’s Human on human IHC kit (AP/Permanent Red) (ab214753) is a ready to use system that results in excellent sensitivity and high specificity using a simple protocol. Furthermore, as the protocol uses a polymer-based, biotin-free system, it does not require blocking of endogenous biotin.
Note: This kit is recommended for cytoplasmic and membrane bound antigens other patterns of staining have not tested.
Tested applicationsSuitable for: IHCmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 6 ml 18 ml 110 ml 100X Permanent Red Chromogen 1 x 70µl 1 x 180µl 1 x 1.2ml 5X Permanent Red Activator 1 x 1.4ml 1 x 3.6ml 2 x 12ml 5X Quenching Buffer 1 x 1.5ml 2 x 2.3ml 2 x 13ml Blocking Buffer A 1 x 6ml 1 x 18ml 1 x 110ml Blocking Buffer B 1 x 6ml 1 x 18ml 1 x 110ml Human AP Polymer 1 x 6ml 1 x 18ml 1 x 110ml Human Primer 1 x 6ml 1 x 18ml 1 x 110ml Permanent Red Substrate 1 x 7ml 1 x 18ml 1 x 110ml
Our Abpromise guarantee covers the use of ab214753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC||Use at an assay dependent concentration.|
ab214753 has not yet been referenced specifically in any publications.