Overview

  • Product name

    Human on human IHC kit (HRP/DAB)
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Human on human IHC kit (HRP/DAB) (ab214749) is designed for staining human primary antibodies on human tissues without background staining.


    Antigen detection using a primary antibody of the same species as the test tissue yields high background when an indirect detection method is used. This severely limits the ability to screen human antibodies on human tissues. Abcam’s Human on human IHC kit (HRP/DAB) (ab214749) is a ready to use system that results in excellent sensitivity and high specificity using a simple protocol. Furthermore, as the protocol uses a polymer-based, biotin-free system, it does not require blocking of endogenous biotin.  


     

  • Tested applications

    Suitable for: IHCmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 6 ml 18 ml 110 ml
    20X DAB Chromogen 1 x 1.5ml 1 x 2ml 1 x 6ml
    5X Quenching Buffer 1 x 1.5ml 2 x 2.3ml 2 x 13ml
    Blocking Buffer A 1 x 6ml 1 x 18ml 1 x 110ml
    Blocking Buffer B 1 x 6ml 1 x 18ml 1 x 110ml
    DAB Substrate Buffer 1 x 12ml 2 x 15ml 1 x 120ml
    Human HRP Polymer 1 x 6ml 1 x 18ml 1 x 110ml
    Human Primer 1 x 6ml 1 x 18ml 1 x 110ml

Associated products

Applications

Our Abpromise guarantee covers the use of ab214749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC Use at an assay dependent concentration.

Protocols

References

ab214749 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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