Overview

  • Product name

    Human Oncostatin M/OSM ELISA Kit
    See all Oncostatin M/OSM kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 7%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    2.1 pg/ml
  • Range

    15.625 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 93 86% - 97%
    Cell culture media 102 88% - 114%
    Heparin Plasma 95 89% - 98%
    EDTA Plasma 85 82% - 90%
    Citrate Plasma 80 78% - 81%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Oncostatin M/OSM in vitro SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Oncostatin M protein in human serum, plasma, and cell culture supernatant. 


    The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Oncostatin M is a 28-kDa pleiotropic cytokine of the IL-6 family that is a product of activated T lymphocytes, monocytes, neutrophils, and some tumor cells including breast cancer epithelial cells. Oncostatin M participates in a number of developmental, skeletal and immunological processes. Oncostatin M inhibits the proliferation of a number of tumor cell lines. It stimulates proliferation of AIDS-KS cells. Oncostatin M regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. It uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Oncostatin M is involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.

     

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab215543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Oncostatin M were measured in duplicates, interpolated from the Oncostatin M standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated U937 supernatant 50% and stimulated PBMC supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean (target) concentration was determined to be 362.5 pg/mL in neat stimulated U937 supernatant and 2800 pg/mL in neat stimulated PBMC supernatant.

  • The concentrations of Oncostatin M were measured in duplicates, interpolated from the Oncostatin M standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • U937 cells were cultured in the absence or presence of 10 ng/mL TPA for 72 hours. The concentrations of Oncostatin M were measured in 50% supernatant samples in duplicates and interpolated from the IL-4 standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean Oncostatin M concentration was determined to be 363 pg/mL in neat TPA stimulated U937 cell supernatant, 24.9 pg/mL in neat unstimulated supernatants and undetectable in media (not shown).

  • Human PBMC cells were cultured in the absence or presence of 1.5% PHA-M for 46 hours. The concentrations of Oncostatin M were measured in 25% supernatant samples in duplicates and interpolated from the Oncostatin M standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean Oncostatin M concentration was determined to be 2800 pg/mL in neat PHA-M stimulated PBMC cell supernatant, 96 pg/mL in neat unstimulated supernatants and undetectable in media (not shown).

Protocols

References

ab215543 has not yet been referenced specifically in any publications.

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