Human Osteoactivin ELISA Kit (ab193711)
- Datasheet
- References
- Protocols
Overview
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Product name
Human Osteoactivin ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
45 pg/ml -
Range
45 pg/ml - 12000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 117.8 111% - 126% Plasma 116.5 107% - 126% Cell culture media 112.9 98% - 149% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Osteoactivin Human ELISA Kit (ab193711) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Osteoactivin in sera, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Osteoactivin coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Osteoactivin present in the samples. The wells are washed and biotinylated anti-Human Osteoactivin antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Osteoactivin bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated Human Osteoactivin detection antibody (lyophilized) 2 vials Human Osteoactivin Standard (lyophilized) 2 vials Pre-coated Human Osteoactivin Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Function
Could be a melanogenic enzyme. -
Tissue specificity
Up-regulated in various cancer cells, including in glioblastoma multiforme. Expressed in many melanoma cells, as well as in tissue macrophages, including liver Kuppfer cells and lung alveolar macrophages, in podocytes and in some cells of the ciliary body of the eye (at protein level). Hardly detectable in healthy brain. -
Involvement in disease
Glioblastoma multiforme patients that exhibit increased mRNA and protein levels (>3-fold over normal brain) in biopsy samples have a significantly higher risk of death (PubMed:16609006). In the context of cancer immunotherapy, including that of melanoma, has been proposed to be used as a cell surface protein antigen targeted by antibodies coupled to cytotoxic drugs (PubMed:16489096). -
Sequence similarities
Belongs to the PMEL/NMB family.
Contains 1 PKD domain. -
Cellular localization
Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Detected at the cell surface in different types of cancers cells, including glioblastoma multiforme cells and most melanoma cell lines. - Information by UniProt
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Alternative names
- Gpnmb
- GPNMB_HUMAN
- Transmembrane glycoprotein HGFIN
- Transmembrane glycoprotein NMB
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Database links
- Entrez Gene: 10457 Human
- Omim: 604368 Human
- SwissProt: Q14956 Human
- Unigene: 190495 Human
Applications
Our Abpromise guarantee covers the use of ab193711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Typical standard curve in Assay Diluent AData provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Typical standard curve in Assay Diluent BData provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Protocols
References
ab193711 has not yet been referenced specifically in any publications.
