Overview

  • Product name

    Human Osteocalcin ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 8 8.54%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 3.73%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.1 ng/ml
  • Range

    0.156 ng/ml - 10 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 117 114% - 122%
    Heparin Plasma 109 101% - 117%
    EDTA Plasma 108 99% - 119%
    Citrate Plasma 111 107% - 117%
    serum free media 97 90% - 102%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human Osteocalcin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Osteocalcin protein in human serum, plasma and serum-free cell culture supernatants. Quantitate Human Osteocalcin with 0.1 ng/ml sensitivity.


    SimpleStep ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA protocol summary in the image section for further details. Our SimpleStep ELISA technology provides several benefits:


      1)  Single-wash protocol reduces assay time to 90 minutes or less


      2)  High sensitivity, specificity and reproducibility from superior antibodies


      3)  Fully validated in biological samples


      4)  96-wells plate breakable into 12 x 8 wells strips

  • Notes

    Osteocalcin is a small secreted protein 49 amino acids produced by osteoblasts. Secreted OCN has post-translational modification of specific glutamic acid residues via carboxylation to form γ-carboxyglutamtic acid (GLA) residues. This modification is thought to increase the affinity of OCN for free mineral ions in serum, forming a complex, and thus becoming absorbed into the bone extracellular matrix (ECM). OCN also acts as a hormone impacting glucose metabolism and insulin secretion. During bone reabsorption osteoclasts break down the bone matrix releasing bound Osteocalcin into 5 different fragments; N-terminal, Mid, C-terminal, N-terminal-Mid, and Mid-C-terminal into the serum. Circulating levels of intact OCN and OCN fragments have been found to vary significantly between healthy individuals and diseased stats such as Paget’s disease, osteoporosis, fracture, and renal failure.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Osteocalcin Capture Antibody 1 x 600µl
    10X Human Osteocalcin Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human Osteocalcin Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Function

    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • Sequence similarities

    Belongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • Post-translational
    modifications

    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • BGLAP
    • BGP
    • Bone gamma carboxyglutamate (gla) protein
    • Bone Gla protein
    • Gamma-carboxyglutamic acid-containing protein
    • OC
    • OCN
    • OSTCN_HUMAN
    • Osteocalcin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab195214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated values of Osteocalcin from serum of 10 male donors are plotted.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Background subtracted data from duplicate measurements are plotted. Measureable levels of Osteocalcin were observed in MG-63 (4-day) serum free supernatants, while no Osteocalcin was detected in HeLa supernatants or serum free media.

Protocols

References

This product has been referenced in:

  • Liu Y  et al. Upregulation of long noncoding RNA MEG3 inhibits the osteogenic differentiation of periodontal ligament cells. J Cell Physiol 234:4617-4626 (2019). Read more (PubMed: 30256394) »
  • Shen YF  et al. Mineral Trioxide Aggregate Mixed with 5-Aminolevulinic Acid for the Photodynamic Antimicrobial Strategy in Hard Tissue Regeneration. Materials (Basel) 11:N/A (2018). Read more (PubMed: 30223515) »
See all 3 Publications for this product

Customer reviews and Q&As

Very quick osteocalcin ELISA

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The kit is extremely easy to use and since there are so few steps (plus pre-coated plates), it takes only 90 minutes.
I used serum samples. Dilution should be at least 1:100 to get OD values within the standard range.

Abcam user community

Verified customer

Submitted Dec 09 2016

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