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Human Osteoprotegerin ELISA Kit (ab100617)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (2)References (6)

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Osteoprotegerin Human ELISA Kit - ab100617
  • Osteoprotegerin Human ELISA Kit	-ab100617
  • Typical Standard Curve
  • Typical Standard Curve

Key features and details

  • Sensitivity: 1 pg/ml
  • Range: 1.23 pg/ml - 900 pg/ml
  • Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human Osteoprotegerin ELISA Kit
    See all Osteoprotegerin kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma, Cell Lysate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 1 pg/ml
  • Range

    1.23 pg/ml - 900 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 99.75 89% - 108%
    Serum 99.85 89% - 108%
    Plasma 102.45 90% - 109%
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Osteoprotegerin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Osteoprotegerin in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human Osteoprotegerin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteoprotegerin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Osteoprotegerin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteoprotegerin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human Osteoprotegerin ELISA Kit (ab189580) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

    We recommend preparing serum-free or low-serum medium samples, as serum tends to contain cytokines which may produce significant background signals. If it is necessary to test serum-containing medium, we recommend also running an uncultured media blank to assess baseline signals. This baseline can then be subtracted from the cultured media sample data.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human Osteoprotegerin 2 vials
    Osteoprotegerin Microplate (12 x 8 wells) 1 unit
    Recombinant Human Osteoprotegerin Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

    • Cardiovascular
    • Blood
    • Other
    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Atherosclerotic proteins ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
    • Metabolism
    • Types of disease
    • Obesity
  • Function

    Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.
  • Tissue specificity

    Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung.
  • Involvement in disease

    Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD) [MIM:239000]; also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast-mediated skeletal resorption, the disease can be fatal.
  • Sequence similarities

    Contains 2 death domains.
    Contains 4 TNFR-Cys repeats.
  • Post-translational
    modifications

    N-glycosylated. Contains sialic acid residues.
    The N-terminus is blocked.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession O00300 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • MGC29565
    • OCIF
    • OPG
    • Osteoclastogenesis inhibitory factor
    • Osteoprotegerin
    • PDB5
    • TNF receptor superfamily member 11b
    • TNFRSF 11B
    • TNFRSF11B
    • TR 1
    • TR1
    • TR11B_HUMAN
    • Tumor necrosis factor receptor superfamily member 11B
    see all
  • Database links

    • Entrez Gene: 4982 Human
    • Omim: 602643 Human
    • SwissProt: O00300 Human
    • Unigene: 81791 Human

    Associated products

    • SimpleStep ELISA kits

      • Human Osteoprotegerin ELISA Kit (ab189580)

    Images

    • Osteoprotegerin Human ELISA Kit - ab100617
      Osteoprotegerin Human ELISA Kit - ab100617
    • Osteoprotegerin Human ELISA Kit	-ab100617
      Osteoprotegerin Human ELISA Kit -ab100617

      Osteoprotegerin measured in cell lysates showing quantity (pg) per mg of tested sample

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab100617.

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab100617.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (6)

    Publishing research using ab100617? Please let us know so that we can cite the reference in this datasheet.

    ab100617 has been referenced in 6 publications.

    • Kim BJ  et al. The Detrimental Effects of Kynurenine, a Tryptophan Metabolite, on Human Bone Metabolism. J Clin Endocrinol Metab N/A:N/A (2019). PubMed: 30715395
    • Kim BJ  et al. Association of blood n-3 fatty acid with bone mass and bone marrow TRAP-5b in the elderly with and without hip fracture. Osteoporos Int N/A:N/A (2019). PubMed: 30719549
    • Kim BJ  et al. Elevated ceramides 18:0 and 24:1 with aging are associated with hip fracture risk through increased bone resorption. Aging (Albany NY) 11:9388-9404 (2019). PubMed: 31675352
    • Sawicka-Powierza J  et al. Bone Metabolism Markers and Bone Mineral Density in Patients on Long-Term Acenocoumarol Treatment: A Cross-Sectional Study. J Clin Med 7:N/A (2018). PubMed: 30347817
    • Stojanovic S  et al. Stem Cells Derived from Lipoma and Adipose Tissue-Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature. Cells 7:N/A (2018). PubMed: 30544806
    • Vincenzi F  et al. Pulsed electromagnetic fields increased the anti-inflammatory effect of A2A and A3 adenosine receptors in human T/C-28a2 chondrocytes and hFOB 1.19 osteoblasts. PLoS One 8:e65561 (2013). Human . PubMed: 23741498

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Thank you for the reply to Olga, I will take over now. Did you also have OPG ELISA kit to offer?

    RANKL (ab100749) seems to be suitable, are you willing to quote in 25 kits? (or 25 plates if you have kits with more than one plate)

    I will also need a quotation in the OPG kit if you can offer that as well. 25 kits (or 25 plates if you have kits with more than one plate).

    Looking forward to your prompt reply,

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 28 2012

    Answer

    Thank you for your email.

    Yes, we do have OPG kits available. The catalogue number is ab100617 and ab100733, one is specific to human and other one to mouse.

    https://www.abcam.com/Osteoprotegerin-Human-ELISA-Kit-1-x-96-Well-Plate-ab100617.html

    https://www.abcam.com/Osteoprotegerin-Mouse-ELISA-Kit-ab100733.html

    My colleague will be sending you the quote for 25 kits soon. Could you confirm which one of these you would choose for quotation?

    Please let me know if you need more information.

    Read More

    Abcam Scientific Support

    Answered on Sep 28 2012

    Question

    I wish to test 400 serum samples using your ELISA kits for: 1. Osteoprotegerin (Catalogue number ab100617) 2. MMP3 (Catalogue number ab100607) 3. DKK1 (Catalogue number ab100501)   I wonder if you could answer a few queries for me: 1. Please could you provide me with a 'Kit Insert' for each kit? - I was unable to find a link on your website. 2. Can I use these specific kits on an automated ELISA machine? - I would prefer not to test 400 samples individually. 3. As I would like to order 6 kits for each biomarker (thus 18 kits in total) would you be able to give me a more competitive rate than that stated onthe website? 4. Some of our serum samples have been stored frozen for 3-5 years. Will this affect the assay?   Would you prefer to discuss during a site visit?   I look forward to hearing from you.  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 18 2011

    Answer

    Thank you for your interest in our kits. I will send the product inserts first thing tomorrow morning, EST. The kits will be compatible with the automated ELISA machine, if the automated set-up approximates the conditions specified in the kit protocols. We provide a discount for orders of 5 or more kits. I will have our customer service department prepare a proforma. I do not anticipate any issues with the age of the sera, if they have been stored at a consistent temperature of -20C or less. You may want to conduct a trial before committing to a large run, however.

    Read More

    Abcam Scientific Support

    Answered on Oct 18 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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