Overview

  • Product name
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Cell medium 5 3.6%
    Inter-assay
    Sample n Mean SD CV%
    Cell medium 3 4.9%
  • Sample type
    Cell culture supernatant, Serum, Cell culture extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    23 pg/ml
  • Range
    46.9 pg/ml - 3000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 120 116% - 122%
    Cell culture extracts 106 102% - 111%
    Cell culture media 113 112% - 114%
    Heparin Plasma 120 116% - 121%
    EDTA Plasma 115 112% - 117%
    Citrate Plasma 116 113% - 117%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    OX40 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of OX40 protein in human serum, plasma, cell culture supernatant, cell and tissue extract.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    OX40 (TNFRSF4) is a single pass type 1 membrane receptor protein for OX40 Ligand and implicated in long-term t-cell immunity.  Binding of OX40 and OX40L enhances cytokine production, T-cell survival and proliferation.  ab231926 is designed to recognize the extracellular domain.


    Sensitivity:


    Samples diluted in Sample Diluent NS - 26 pg/mL


    Samples diluted in 1X Cell Extraction Buffer PTR - 23 pg/mL

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab231926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • ELISA Protocol Summary
  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of OX40 were measured in duplicates, interpolated from the OX40 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 25%, plasma (heparin) 12.5% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    Serum from ten individual healthy human female donors was measured in duplicate. No detectable levels of OX40 were measured.

  • The concentrations of OX40 were measured in duplicates, interpolated from the OX40 standard curves and corrected for sample dilution. Undiluted samples are as follows: Stimulated PBMC cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OX40 concentration was determined to be 296 pg/mL in Stimulated PBMC cell culture supernatant, and undetectable in unstimulated and media controls.

  • The concentrations of OX40 were measured in duplicate and interpolated from the OX40 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OX40 concentration was determined to be 419 pg/mL in Jurkat Cell Extract.

Protocols

References

ab231926 has not yet been referenced specifically in any publications.

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