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We are planning to analyse EGFR and P53 by blood serum of lung cancer patient with Elisa. My query regarding this is whether you cater to these kind of kits? If yes what will be the amount for 100 patients? We had done EGFR and p53 earlier with IHC on tumor tissue. As this will be a new protocol whether you will support tostandardize this assay in our lab.
Asked on May 14 2012
Thank you for contacting us.
We have two P53 related kits in our catalogue ab46067 and ab46113. These kids have been tested and fully guaranteed to be used with human serum samples. In any case if these does not work we will be happy to provide troubleshooting suggestions. Please the Abpromise on our website;
The protocol suggest using 100ul of sample per well, so for duplicate in a serial dilution you would need 400ul of serum.
The EGFR kits we have is ab100505
Again this kit is fully guaranteed and 100 ul sample per well is needed.
The number of kits you would need totally depends on the assay you device. These kits have enough material for 96 wells ELISA plate. The 96 wells can help in calculating the number of kits you would need for 100 patients. My suggestions would be
8x2 = 16 (standard in duplicate, serial diluted)
8x2 = 16 (Sample in duplicate, serially diluted, patient 1)
the rest 64 wells can be used for 4 patients in duplicate (16 wells per sample) or for 8 patients. It totally depends on how you set up your experiment.
We will be happy to troubleshoot the protocol if these kits do not perform as suggest in the protocol, provided with the datasheet.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Answered on May 14 2012