• Product name
    Human PAI1 ELISA Kit (SERPINE1)
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall 4.8%
    Sample n Mean SD CV%
    Overall 9.6%
  • Sample type
    Cell culture supernatant, Serum, Plasma, Tissue, Cerebral Spinal Fluid
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 0.02 ng/ml
  • Range
    0.078 ng/ml - 5 ng/ml
  • Recovery

    98 %

  • Assay time
    4h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s PAI1 (SERPINE1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 concentrations in Human plasma, serum, tissue extracts and cell culture supernatants.

    A PAI1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PAI1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PAI1 captured in plate.

    This assay recognizes both natural and recombinant Human PAI-1.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    1X Standard Diluent 1 x 2ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human PAI1 Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    PAI1 Microplate (12 x 8 well strips) 1 unit
    PAI1 Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.
  • Tissue specificity
    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • Involvement in disease
    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • Sequence similarities
    Belongs to the serpin family.
  • Post-translational
    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • Endothelial plasminogen activator inhibitor
    • PAI
    • PAI-1
    • PAI1_HUMAN
    • Plasminogen activator inhibitor 1
    • Serpin E1
    • SERPINE1
    see all
  • Database links


Our Abpromise guarantee covers the use of ab108891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.


  • Standard curve mean of duplicates (+/- SD) with background readings subtracted

  • PAI1 measured in various samples showing quantity (ng) per mL of tested sample

  • PAI1 measured in cell lysates showing quantity (ng) per 1 million cells tested



This product has been referenced in:
  • Giacoia EG  et al. PAI-1 leads to G1-phase cell-cycle progression through cyclin D3/cdk4/6 upregulation. Mol Cancer Res 12:322-34 (2014). Read more (PubMed: 24464915) »
  • Jin R  et al. Dietary fructose reduction improves markers of cardiovascular disease risk in Hispanic-American adolescents with NAFLD. Nutrients 6:3187-201 (2014). Human . Read more (PubMed: 25111123) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

I can confirm that Heparin should not be interfering with the assay using these kits.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or informat...

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Thank you for contacting us.

I checked with the lab and they let me know that the homology with mouse is only 2%, thus they do not recommend using it for this species. It will most likely not work.

Let me know if I can assist you f...

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Thank you for contacting Abcam regarding ab108891.

Our human kit is specific for human PAI-1 total antigen measurement. It does not cross react with rabbit PAI-1. Unfortunately, we do not have a rabbit specific kit available.

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Thank you for your enquiry.

This kit measures the total PAi-1 including free PAI-1, PAI-tPA complexes and PAI-1-uPA complexes.

I hope this information will be helpful.

Thank you for contacting us.

All of the kits mentioned (ab46027, ab100512, ab100640, ab108891) are suitable for use with citrate plasma.

I hope this information has been of help. If your require any further information please do not...

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Thank you for contacting Abcam today.

I am sorry about the confusion over the labeling of the standards for ab108891 and ab108914. I have heard back from the lab about the correct concentrations for these standards:

ab108891 - Recon...

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