Product nameAnti-Human Papillomavirus 16 (E7) antibody [289-17013]
See all Human Papillomavirus 16 (E7) primary antibodies
DescriptionMouse monoclonal [289-17013] to Human Papillomavirus 16 (E7)
Tested applicationsSuitable for: IP, WB, ELISA, RIA, Flow Cyt, IHC-Frmore details
Species reactivityReacts with: Other species
Full length protein corresponding to Human Papillomavirus 16 (E7). Immunogen: HPV 16 E7 synthesized in S. pombe.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.87% Sodium chloride, PBS
Concentration information loading...
Purification notesPurified from ascites.
Our Abpromise guarantee covers the use of ab30731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 11 kDa. Dilution optimised using Chromogenic detection.|
|ELISA||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
RelevanceE7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription
- E7 antibody
- HPV16 E7 antibody
- Protein E7 antibody
- Transforming protein antibody
This product has been referenced in:
- Liu Y et al. Nuclear lactate dehydrogenase A senses ROS to produce a-hydroxybutyrate for HPV-induced cervical tumor growth. Nat Commun 9:4429 (2018). Read more (PubMed: 30356100) »
- Mitkevich VA et al. Binase treatment increases interferon sensitivity and apoptosis in SiHa cervical carcinoma cells by downregulating E6 and E7 human papilloma virus oncoproteins. Oncotarget 8:72666-72675 (2017). Read more (PubMed: 29069817) »